PXD026353

PXD026353 is an original dataset announced via ProteomeXchange.

Title	SCFFbxw5 targets kinesin-13 proteins to facilitate ciliogenesis
Description	Using substrate screening on protein microarrays we identified the kinesin-13 Kif2c/MCAK as a novel target of the ubiquitin E3 ligase SCF-Fbxw5. In cells, SCF-Fbxw5 ubiquitylates MCAK for proteasomal degradation during G2/M thereby facilitating ciliogenesis in the following G0 phase. Here, we performed mass spectrometry analysis to identify lysine residues of recombinant MCAK that are specifically ubiquitylated in vitro by SCF-Fbxw5 in concert with the E2 enzyme Cdc34 based on diGly remnants after trypsin digestion.
HostingRepository	PRIDE
AnnounceDate	2021-08-13
AnnouncementXML	Submission_2021-08-12_21:40:48.893.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Ruppert
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitination signature dipeptidyl lysine
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-28 09:00:19	ID requested
1	2021-08-11 23:52:32	announced
⏵ 2	2021-08-12 21:40:49	announced	2021-08-13: Updated project metadata.

10.15252/EMBJ.2021107735;

Schweiggert J, Habeck G, Hess S, Mikus F, Beloshistov R, Meese K, Hata S, Knobeloch KP, Melchior F, targets kinesin-13 proteins to facilitate ciliogenesis. EMBO J, 40(18):e107735(2021) [pubmed]

submitter keyword: Cullin-RING ligase / ubiquitin / Fbxw5 / MCAK / cilia

Thomas Ruppert
contact affiliation	ZMBH University Heidelberg Im Neuenheimer Feld 282 69120 Heidelberg Germany
contact email	t.ruppert@zmbh.uni-heidelberg.de
lab head
Thomas Ruppert
contact affiliation	ZMBH, Im Neuenheimer Feld 282, 69122 Heidelberg
contact email	t.ruppert@zmbh.uni-heidelberg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD026353

PRIDE project URI

• PRIDE
  1. PXD026353
     1. Label: PRIDE project
     2. Name: SCFFbxw5 targets kinesin-13 proteins to facilitate ciliogenesis