PXD026352

PXD026352 is an original dataset announced via ProteomeXchange.

Title	DNA-PKcs kinase activity stabilizes the transcription factor Egr1 in activated immune cells
Description	To gain insight into the function of DNA-PKcs within immune cells, we performed a quantitative phosphoproteomic screen in T cells to identify first order phosphorylation targets of DNA-PKcs. Results indicate that DNA-PKcs phosphorylates the transcription factor Egr1 (early growth response protein 1) at S301. Expression of Egr1 is induced early upon T cell activation and dictates T cell response by modulating expression of cytokines and key costimulatory molecules. Mutation of serine 301 to alanine via CRISPR-Cas9 resulted in increased proteasomal degradation of Egr1 and a decrease in Egr1-dependent transcription of IL2 (interleukin-2) in activated T cells. Our findings identify DNA-PKcs as a critical intermediary link between T cell activation and T cell fate and a novel phosphosite involved in regulating Egr1 activity.
HostingRepository	PRIDE
AnnounceDate	2021-12-21
AnnouncementXML	Submission_2021-12-21_03:35:20.950.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aaron Storey
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-28 09:00:12	ID requested
1	2021-12-21 02:53:54	announced
⏵ 2	2021-12-21 03:35:21	announced	2021-12-21: Updated publication reference for PubMed record(s): 34562454.

Waldrip ZJ, Burdine L, Harrison DK, Azevedo-Pouly AC, Storey AJ, Moffett OG, Mackintosh SG, Burdine MS, DNA-PKcs kinase activity stabilizes the transcription factor Egr1 in activated immune cells. J Biol Chem, 297(4):101209(2021) [pubmed]

submitter keyword: TMT, MS3, Phospo

Marie Burdine
contact affiliation	Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, AR
contact email	MBurdine@uams.edu
lab head
Aaron Storey
contact affiliation	University of Arkansas for Medical Sciences
contact email	ajstorey@uams.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026352
     1. Label: PRIDE project
     2. Name: DNA-PKcs kinase activity stabilizes the transcription factor Egr1 in activated immune cells