PXD026317 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | G-CSF mediated signalling in 32D cells |
| Description | G-CSF signalling has been extensively characterized in myeloid progenitor cells but the activation dynamics downstream of the receptor at different stages of cell differentiation in wild-type G-CSFR or in proliferating cells that harbor the d715 mutation has not been been investigated at the phosphoproteome/proteome level. Proteome and phosphoproteome profiling using 10 plexTMT has been performed on 32D cells stimulated with G-CSF on d715 mutant cells, wild type cells on day 0, day 3 and day 6 of cell differentiation. Each sample on each day has 5 time points sampled at t=0,5,10,15,30 minutes post G-CSF stimulation. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-22 |
| AnnouncementXML | Submission_2026-06-22_09:08:03.585.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | James Wright |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
| 0 | 2021-05-27 08:07:16 | ID requested | |
| ⏵ 1 | 2026-06-22 09:08:04 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: TMT,32D,phosphoproteom,proteome |
Contact List
| Jyoti Choudhary |
| contact affiliation | The Institute of Cancer Research |
| contact email | jyoti.choudhary@icr.ac.uk |
| lab head | |
| James Wright |
| contact affiliation | The Institute of Cancer Research |
| contact email | james.wright@icr.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD026317
- Label: PRIDE project
- Name: G-CSF mediated signalling in 32D cells