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PXD026317

PXD026317 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleG-CSF mediated signalling in 32D cells
DescriptionG-CSF signalling has been extensively characterized in myeloid progenitor cells but the activation dynamics downstream of the receptor at different stages of cell differentiation in wild-type G-CSFR or in proliferating cells that harbor the d715 mutation has not been been investigated at the phosphoproteome/proteome level. Proteome and phosphoproteome profiling using 10 plexTMT has been performed on 32D cells stimulated with G-CSF on d715 mutant cells, wild type cells on day 0, day 3 and day 6 of cell differentiation. Each sample on each day has 5 time points sampled at t=0,5,10,15,30 minutes post G-CSF stimulation.
HostingRepositoryPRIDE
AnnounceDate2026-06-22
AnnouncementXMLSubmission_2026-06-22_09:08:03.585.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJames Wright
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-05-27 08:07:16ID requested
12026-06-22 09:08:04announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: TMT,32D,phosphoproteom,proteome
Contact List
Jyoti Choudhary
contact affiliationThe Institute of Cancer Research
contact emailjyoti.choudhary@icr.ac.uk
lab head
James Wright
contact affiliationThe Institute of Cancer Research
contact emailjames.wright@icr.ac.uk
dataset submitter
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Dataset FTP location
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