PXD026316

PXD026316 is an original dataset announced via ProteomeXchange.

Title	Label-free quantitative proteomic analysis for identification of in-planta Magnaporthe oryzae proteins induced cell death
Description	The rice blast disease caused by Magnaporthe oryzae (M.oryzae) is one of the most important disease in cultivated rice (Oryza sativa L.) results in extensive loss of productivity worldwide. Therefore, here we utilized the label-free quantitative proteomic approach to identify the novel M. oryzae effectors. A total of 114 were significantly modulated M. oryzae proteins derived from infected rice leaves. Among them, twenty-nine proteins were predicted as secreted proteins by SignalP 4.0. In addition, functional annotation proteins revealed that five proteins were mainly associated with hydrolase family. Consequently, five putative effector genes were cloned into a plant expression vector and fused with MYC tag in the C-terminal. Finally, we found that three in-planta M.oryzae hydrolase proteins were induced cell death after Agrobacterium tumefaciens-mediated transient expression in Nicotiana benthamiana (N.benthamiana). MGP1 (endo-1,4-beta-xylanase) and MGP4 (cellulose-binding protein) were localized in extracellular. Otherwise, MGP5 (cutinase) localized in intracellular space induced cell death. Thus, our results suggest that in-planta M. oryzae hydrolase proteins may play a key role in innate immunity via PAMP (Pathogen-Associated Molecular Pattern) in rice.
HostingRepository	PRIDE
AnnounceDate	2022-05-27
AnnouncementXML	Submission_2022-05-27_02:51:31.435.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sun Tae Kim
SpeciesList	scientific name: Oryza sativa Japonica Group; NCBI TaxID: 39947;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-27 08:04:09	ID requested
⏵ 1	2022-05-27 02:51:32	announced

Jeon J, Kim KT, Choi J, Cheong K, Ko J, Choi G, Lee H, Lee GW, Park SY, Kim S, Kim ST, Min CW, Kang S, Lee YH, Alternative splicing diversifies the transcriptome and proteome of the rice blast fungus during host infection. RNA Biol, 19(1):373-385(2022) [pubmed]

submitter keyword: Magnaporthe oryzae, Oryza sativa, Label-free, SignalP, Nicotiana benthamiana, MaxQuant, Perseus

Sun Tae Kim
contact affiliation	Department of Plant Bioscience, Life and industry Convergence Research Institute, Pusan National University, Miryang 50463, South Korea
contact email	stkm5505@gmail.com
lab head
Sun Tae Kim
contact affiliation	Pusan National University
contact email	stkim5505@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026316
     1. Label: PRIDE project
     2. Name: Label-free quantitative proteomic analysis for identification of in-planta Magnaporthe oryzae proteins induced cell death