PXD026311

PXD026311 is an original dataset announced via ProteomeXchange.

Title	Alternative oxidase (AOX) seems to play a key role in embryogenic competence acquisition in Carica papaya L. calli
Description	Understanding the mechanisms that endow a somatic cell with the ability to differentiate into a somatic embryo, which could result in numerous biotechnological applications, is still a challenge. The objective of this work was to identify some of the molecular and physiological mechanisms responsible for the acquisition of embryogenic competence during somatic embryogenesis in Carica papaya L. We performed a broad characterization of embryogenic (ECs) and nonembryogenic calli (NECs) of C. papaya using global and mitochondrial proteomic approaches, histomorphology, histochemistry, respiratory activity, and endogenous hormonal and hydrogen peroxide contents. ECs and NECs presented remarkable differences in anatomical and histochemical characteristics. ECs showed greater reactivity for the presence of proteins and neutral polysaccharides. Our results demonstrate the role of mitochondrial metabolism in the embryogenic competence of C. papaya calli. Greater participation of alternative oxidase (AOX) enzymes in respiration, as well as stronger accumulation of mitochondrial stress response proteins, was observed in ECs. In addition, ECs showed a greater abundance of proteins related to oxidative phosphorylation and higher total respiration (TR). Auxin-responsive Gretchen Hagen 3 (GH3) family proteins may play an important role in decreasing the contents of free 2,4-dichlorophenoxyacetic acid (2,4-D) in ECs. The accumulation of stress response proteins among total proteins was observed in ECs. ECs also showed higher endogenous hydrogen peroxide (H2O2) contents. H2O2 is a promising molecule for further investigation in differentiation protocols for C. papaya somatic embryos.
HostingRepository	PRIDE
AnnounceDate	2022-06-28
AnnouncementXML	Submission_2022-06-27_20:20:02.455.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vanildo Silveira
SpeciesList	scientific name: Carica papaya; NCBI TaxID: 3649;
ModificationList	No PTMs are included in the dataset
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-27 08:03:40	ID requested
⏵ 1	2022-06-27 20:20:03	announced

Vale EM, Santana DB, Reis RS, Sousa KR, de Souza Filho GA, Oliveira JG, Santa-Catarina C, Silveira V, Mitochondrial proteomics reveals new insights into embryogenic competence acquisition in Carica papaya L. callus. J Proteomics, 252():104434(2022) [pubmed]

submitter keyword: Hormones, Label-free proteomics, Mitochondrial proteomics, Somatic embryos

Vanildo Silveira
contact affiliation	Laboratório de Biotecnologia, Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Av. Alberto Lamego, Campos dos Goytacazes, RJ, Brazil. Unidade de Biologia Integrativa, Setor de Genômica e Proteômica, UENF, Campos dos Goytacazes, RJ, Brazil.
contact email	vanildo@uenf.br
lab head
Vanildo Silveira
contact affiliation	UNIVERSIDADE ESTADUAL DO NORTE FLUMINENSE
contact email	vanildo@uenf.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026311
     1. Label: PRIDE project
     2. Name: Alternative oxidase (AOX) seems to play a key role in embryogenic competence acquisition in Carica papaya L. calli