PXD026303

PXD026303 is an original dataset announced via ProteomeXchange.

Title	Aorta-on-a-chip reveals impaired mitochondrial dynamics as a therapeutic target for aortic aneurysm in bicuspid aortic valve disease
Description	Bicuspid aortic valve (BAV) is the most common congenital cardiovascular disease in general population and is frequently associated with the development of thoracic aortic aneurysm (TAA). There is no effective strategy to intervene with TAA progression due to an incomplete understanding of the pathogenesis. In this study, protein analyses of human aortic tissues showed the insufficient expression of NOTCH1 and impaired mitochondrial dynamics in BAV-TAA. To verify it, we constructed aorta-on-a-chip to replicate the rhythmic tensile on human aorta, on which human aortic smooth muscle cells (HAoSMCs) endured a microenvironment of biomimetic strain unattainable in animal models. HAoSMCs with NOTCH1-knockdown exhibited reduced contractile phenotype and were accompanied by attenuated mitochondrial fusion. Furthermore, we identified that mitochondrial fusion activators (leflunomide and teriflunomide) or mitochondrial fission inhibitor (Mdivi-1) rescued the mitochondrial dysfunction in HAoSMCs from BAV-TAA patients. These findings suggest that impaired mitochondrial dynamics could be a potential therapeutic target for BAV-TAA.
HostingRepository	PRIDE
AnnounceDate	2021-09-11
AnnouncementXML	Submission_2021-09-21_22:57:37.915.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhang Weijia
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-27 02:37:21	ID requested
1	2021-09-10 19:27:47	announced
⏵ 2	2021-09-21 22:57:38	announced	2021-09-22: Updated publication reference for PubMed record(s): 34486519.

Abudupataer M, Zhu S, Yan S, Xu K, Zhang J, Luo S, Ma W, Alam MF, Tang Y, Huang H, Chen N, Wang L, Yan G, Li J, Lai H, Wang C, Zhu K, Zhang W, Aorta smooth muscle-on-a-chip reveals impaired mitochondrial dynamics as a therapeutic target for aortic aneurysm in bicuspid aortic valve disease. Elife, 10():(2021) [pubmed]

submitter keyword: organ-on-a-chip, thoracic aortic aneurysm, bicuspid aortic valve, mitochondrial dynamics, human aortic smooth muscle cells

Weijia Zhang
contact affiliation	Fudan University
contact email	weijiazhang@fudan.edu.cn
lab head
Zhang Weijia
contact affiliation	Fudan University
contact email	weijiazhang@fudan.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026303
     1. Label: PRIDE project
     2. Name: Aorta-on-a-chip reveals impaired mitochondrial dynamics as a therapeutic target for aortic aneurysm in bicuspid aortic valve disease