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PXD026296

PXD026296 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAdverse stem cell features in a single AML sample predict clinical outcome in AML patient cohorts
DescriptionPatients with acute myeloid leukemia (AML) suffer dismal prognosis and the most adverse subpopulation within each tumor determines patient’s prognosis. To better understand challenging features in AML, we studied individual stem cells from a single AML sample, complementing genomic with in vivo functional studies. Primary tumor cells from an AML patient’s first and second relapse were transplanted into NSG mice to establish serially transplantable patient derived xenografts (PDX). In an innovative approach, twelve derivative PDX clones were generated thereof, each derived from a single AML stem cell as proven by molecular barcoding, and were color-marked to facilitate multiplex competitive in vivo assays. PDX clones consisted of four different genomic clusters; one cluster displayed resistance against Cytarabine treatment, while two other clusters harbored increased stem cell potential, indicating that stemness and treatment resistance had evolved independently in the sample. In vivo functional data correlated closely with the phylogenetic tree calculated from exome data. The Cytarabine-resistant cluster was characterized by a distinct gene expression profile, and a score thereof predicted outcome in large clinical patient data cohorts. Taken together, we provide proof of concept that intra-sample heterogeneity mimics inter-sample heterogeneity in AML. Stem cell disparities within a single sample allow insights into adverse characteristics of general importance for AML.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:37:02.261.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAshokkumar Jayavelu
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-05-26 23:39:11ID requested
12022-05-25 09:12:14announced
22024-10-22 05:37:03announced2024-10-22: Updated project metadata.
Publication List
10.1186/s13045-022-01232-4;
Zeller C, Richter D, Jurinovic V, Valtierra-Guti, é, rrez IA, Jayavelu AK, Mann M, Bagnoli JW, Hellmann I, Herold T, Enard W, Vick B, Jeremias I, Adverse stem cell clones within a single patient's tumor predict clinical outcome in AML patients. J Hematol Oncol, 15(1):25(2022) [pubmed]
Keyword List
submitter keyword: AML, Resistance, Proteome, Stem Cells, PDX, Transcriptome
Contact List
Prof.Dr.Matthias Mann
contact affiliationMaxPlanck Institute of Biochemistry
contact emailmmann@biochem.mpg.de
lab head
Ashokkumar Jayavelu
contact affiliationDept Proteomics and Signal Transduction, MaxPlanck Institute of Biochemistry
contact emailjayavelu@biochem.mpg.de
dataset submitter
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Dataset FTP location
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