PXD026296 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Adverse stem cell features in a single AML sample predict clinical outcome in AML patient cohorts |
Description | Patients with acute myeloid leukemia (AML) suffer dismal prognosis and the most adverse subpopulation within each tumor determines patient’s prognosis. To better understand challenging features in AML, we studied individual stem cells from a single AML sample, complementing genomic with in vivo functional studies. Primary tumor cells from an AML patient’s first and second relapse were transplanted into NSG mice to establish serially transplantable patient derived xenografts (PDX). In an innovative approach, twelve derivative PDX clones were generated thereof, each derived from a single AML stem cell as proven by molecular barcoding, and were color-marked to facilitate multiplex competitive in vivo assays. PDX clones consisted of four different genomic clusters; one cluster displayed resistance against Cytarabine treatment, while two other clusters harbored increased stem cell potential, indicating that stemness and treatment resistance had evolved independently in the sample. In vivo functional data correlated closely with the phylogenetic tree calculated from exome data. The Cytarabine-resistant cluster was characterized by a distinct gene expression profile, and a score thereof predicted outcome in large clinical patient data cohorts. Taken together, we provide proof of concept that intra-sample heterogeneity mimics inter-sample heterogeneity in AML. Stem cell disparities within a single sample allow insights into adverse characteristics of general importance for AML. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:37:02.261.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ashokkumar Jayavelu |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-05-26 23:39:11 | ID requested | |
1 | 2022-05-25 09:12:14 | announced | |
⏵ 2 | 2024-10-22 05:37:03 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1186/s13045-022-01232-4; |
Zeller C, Richter D, Jurinovic V, Valtierra-Guti, é, rrez IA, Jayavelu AK, Mann M, Bagnoli JW, Hellmann I, Herold T, Enard W, Vick B, Jeremias I, Adverse stem cell clones within a single patient's tumor predict clinical outcome in AML patients. J Hematol Oncol, 15(1):25(2022) [pubmed] |
Keyword List
submitter keyword: AML, Resistance, Proteome, Stem Cells, PDX, Transcriptome |
Contact List
Prof.Dr.Matthias Mann |
contact affiliation | MaxPlanck Institute of Biochemistry |
contact email | mmann@biochem.mpg.de |
lab head | |
Ashokkumar Jayavelu |
contact affiliation | Dept Proteomics and Signal Transduction, MaxPlanck Institute of Biochemistry |
contact email | jayavelu@biochem.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD026296
- Label: PRIDE project
- Name: Adverse stem cell features in a single AML sample predict clinical outcome in AML patient cohorts