PXD026287

PXD026287 is an original dataset announced via ProteomeXchange.

Title	Two zinc finger proteins with functions in m6A writing interact with HAKAI
Description	The methyltransferase complex (m6A writer), which catalyzes the deposition of N6-methyladenosine (m6A) in mRNAs, is highly conserved across most eukaryotic organisms, but its components and interactions between them are still far from fully understood. Using in vivo interaction proteomics, two HAKAI-interacting zinc finger proteins, HIZ1 and HIZ2, were discovered as novel components of the Arabidopsis m6A writer complex. HAKAI is required for the interaction between HIZ1 and MTA. Whilst HIZ1 knockout plants have normal levels of m6A, plants in which it is overexpressed show reduced methylation. In addition, HIZ1 was found to be involved in root hair development upon auxin transport inhibition in a HAKAI-dependent manner. Mutant plants lacking HIZ2 are viable but have an 85% reduction in m6A abundance and show severe developmental defects. Our findings suggest that HIZ1 appears to be a HAKAI-dependent negative regulator of m6A deposition and HIZ2 is a novel and essential member of the Arabidopsis m6A writer complex.
HostingRepository	PRIDE
AnnounceDate	2022-03-14
AnnouncementXML	Submission_2022-03-23_15:09:10.455.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mi Zhang
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-26 06:00:34	ID requested
1	2022-02-09 02:54:54	announced
2	2022-03-14 08:02:44	announced	2022-03-14: Updated publication reference for PubMed record(s): 35236848.
3	2022-03-14 09:34:11	announced	2022-03-14: Updated project metadata.
⏵ 4	2022-03-23 15:09:11	announced	2022-03-14: Updated project metadata. 2022-03-23: Updated publication reference for PubMed record(s): 35236848.

Zhang M, Bodi Z, Mackinnon K, Zhong S, Archer N, Mongan NP, Simpson GG, Fray RG, A writing interact with HAKAI. Nat Commun, 13(1):1127(2022) [pubmed]

submitter keyword: Arabidopsis thaliana, zinc finger proteins, in vivo crosslinking, HAKAI, N6-methyladenosine (m6A)

Rupert G. Fray
contact affiliation	Room C33 Plant Sciences Sutton Bonington Campus, the University of Nottingham Sutton Bonington Leicestershire LE12 5RD UK
contact email	rupert.fray@nottingham.ac.uk
lab head
Mi Zhang
contact affiliation	The University of Nottingham
contact email	mi.zhang@nottingham.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD026287

PRIDE project URI

• PRIDE
  1. PXD026287
     1. Label: PRIDE project
     2. Name: Two zinc finger proteins with functions in m6A writing interact with HAKAI