PXD026273 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A SISCAPA-based approach for detection of SARS-CoV-2 viral antigens from clinical samples. |
Description | SARS-CoV-2 has created a global disease burden infecting >100 million humans in just over a year. In this study, we adopted a SISCAPA-based enrichment approach using anti-peptide antibodies generated against peptides from the nucleocapsid protein of SARS-CoV-2. We developed a targeted workflow in which nasopharyngeal samples were digested followed by enrichment of viral peptides using the anti-peptide antibodies and targeted parallel reaction monitoring (PRM) analysis using a high-resolution mass spectrometer. This workflow was applied to 41 RT-PCR-confirmed clinical nasopharyngeal swab samples and 30 negative samples. The SISCAPA-based platform described in the current study can serve as one of the alternative methods for SARS-CoV-2 viral detection. |
HostingRepository | PRIDE |
AnnounceDate | 2022-06-03 |
AnnouncementXML | Submission_2022-06-03_13:46:54.369.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Akhilesh Pandey |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-05-26 04:42:32 | ID requested | |
⏵ 1 | 2022-06-03 13:46:55 | announced | |
Publication List
Mangalaparthi KK, Chavan S, Madugundu AK, Renuse S, Vanderboom PM, Maus AD, Kemp J, Kipp BR, Grebe SK, Singh RJ, Pandey A, Correction: A SISCAPA-based approach for detection of SARS-CoV-2 viral antigens from clinical samples. Clin Proteomics, 19(1):11(2022) [pubmed] |
Keyword List
ProteomeXchange project tag: Covid-19 |
submitter keyword: SARS-CoV-2, SISCAPA, Mass spectrometry, Parallel reaction monitoring (PRM) |
Contact List
Akhilesh Pandey |
contact affiliation | Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905 Department of Laboratory Medicine and Pathology & Center for Individualized Medicine Mayo Clinic Rochester MN 55905, USA (Lab head) |
contact email | pandey.akhilesh@mayo.edu |
lab head | |
Akhilesh Pandey |
contact affiliation | Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905 |
contact email | pandey.akhilesh@mayo.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD026273 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD026273
- Label: PRIDE project
- Name: A SISCAPA-based approach for detection of SARS-CoV-2 viral antigens from clinical samples.