PXD026246

PXD026246 is an original dataset announced via ProteomeXchange.

Title	Identification of a host protein interacting with Tomato spotted wilt virus ribonucleoproteins from Nicotiana benthamiana and yeast.
Description	Viruses are obligate intracellular pathogens that depend on host factors to complete their infection cycle. Very little is known of which plant factors are required for successful Tomato spotted wilt orthotospovirus (TSWV) infection. The viral ribonucleoprotein (RNP) fraction from TSWV infected Nicotiana benthamiana plants was purified and its protein composition was analysed by proteomics by mass spectrometry to identify host proteins that co-purify with viral RNPs. Related, we expressed a TSWV replicon system in a non-host system, Bakers’ yeast (Saccharomyces cerevisiae), and purified as well the RNP fraction from yeast. Comparative proteomics was used to find common enriched proteins observed in both yeast and plant RNP fractions.
HostingRepository	PRIDE
AnnounceDate	2021-11-10
AnnouncementXML	Submission_2021-11-10_07:40:44.040.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sjef Boeren
SpeciesList	scientific name: Tomato spotted wilt tospovirus; NCBI TaxID: 1933298; scientific name: Nicotiana benthamiana; NCBI TaxID: 4100; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	S-carboxamidoethyl-L-cysteine; acetylated residue; monohydroxylated residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-25 02:11:29	ID requested
⏵ 1	2021-11-10 07:40:44	announced

Dataset with its publication pending

submitter keyword: eukaryotic translation Elongation Factor 1A (eEF1A), host factor

pro-viral factor, Ribonucleoproteins

RNP

susceptibility factor Tomato spotted wilt orthotospovirus (TSWV)

Sjef Boeren
contact affiliation	Wageningen University
contact email	sjef.boeren@wur.nl
lab head
Sjef Boeren
contact affiliation	Biochemistry
contact email	sjef.boeren@wur.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026246
     1. Label: PRIDE project
     2. Name: Identification of a host protein interacting with Tomato spotted wilt virus ribonucleoproteins from Nicotiana benthamiana and yeast.