PXD026239 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteins that co-purify with tandem affinity-purified PRC5 in Trypanosoma brucei |
Description | The project was to determine the role of a trypanosomatid protein, termed PRC5, in pre-mRNA splicing. PRC5 has no homologs in model organisms but was recurrently co-purified with known splicing factors in Trypanosoma brucei. To determine interacting proteins, PRC5 was C-terminally TAP-tagged (we used the PTP tag) and tandem affinity-purified, and co-purifying proteins identified after separation by SDS-PAGE and in-gel trypsin digest by LC/MS/MS. Many of the enriched proteins were known splicing factors, and sucrose gradient sedimentation identified a complex of five subunits, termed PRP19-related complex or PRC. |
HostingRepository | PRIDE |
AnnounceDate | 2021-12-15 |
AnnouncementXML | Submission_2021-12-15_07:44:20.601.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Arthur Gunzl |
SpeciesList | scientific name: Trypanosoma brucei; NCBI TaxID: 5691; |
ModificationList | S-carboxamidoethyl-L-cysteine; monohydroxylated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-05-24 21:57:11 | ID requested | |
⏵ 1 | 2021-12-15 07:44:21 | announced | |
Publication List
Srivastava A, Ambr, ó, sio DL, Tasak M, Gosavi U, G, ü, nzl A, A distinct complex of PRP19-related and trypanosomatid-specific proteins is required for pre-mRNA splicing in trypanosomes. Nucleic Acids Res, 49(22):12929-12942(2021) [pubmed] |
Keyword List
submitter keyword: Trypanosome, spliceosome, PRP19-related complex, Tandem affinity purification, LC/MS/MS |
Contact List
Arthur Gunzl |
contact affiliation | Department of Genetics and Genome Sciences University of Connecticut Health Center 400 Farmington Avenue Farmington, CT 06030-6403 USA Phone (860) 679-8878 Fax (860) 679-8345 E-mail gunzl@uchc.edu |
contact email | gunzl@uchc.edu |
lab head | |
Arthur Gunzl |
contact affiliation | UConn Health |
contact email | gunzl@uchc.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD026239
- Label: PRIDE project
- Name: Proteins that co-purify with tandem affinity-purified PRC5 in Trypanosoma brucei