PXD026229-1

PXD026229 is an original dataset announced via ProteomeXchange.

Title	Axonal and somatodendritic proteomes of dopamine neurons in the mouse brain
Description	Dopamine (DA) neurons modulate neural circuits and behaviors via dopamine release from expansive, long range axonal projections. The elaborate cytoarchitecture of DA neurons is embedded within complex brain tissues, making it difficult to access the DA neuronal proteome using conventional methods. Here, we demonstrate APEX2 proximity labeling within genetically targeted neurons in the mouse brain, enabling subcellular proteomics with cell type-specificity. By combining APEX2 biotinylation with mass spectrometry, we mapped the somatodendritic and axonal proteomes of DA neurons. Our dataset reveals the proteomic architecture underlying axonal transport, dopamine transmission, and axonal metabolism in DA neurons. We find a significant enrichment of proteins encoded by Parkinson’s disease-linked genes in dopaminergic axons, including proteins with previously undescribed axonal localization. Our proteomic datasets comprise a significant resource for axonal and DA neuronal cell biology, while the methodology developed here will enable future studies of other neural cell types.
HostingRepository	PRIDE
AnnounceDate	2021-06-01
AnnouncementXML	Submission_2021-06-01_08:41:24.176.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter Sims
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-24 10:21:17	ID requested
⏵ 1	2021-06-01 08:41:24	announced

Dataset with its publication pending

submitter keyword: proteomics, APEX2, dopamine neurons, axon

Peter Sims
contact affiliation	Columbia University Irving Medical Center
contact email	pas2182@columbia.edu
lab head
Peter Sims
contact affiliation	Columbia University
contact email	pas2182@columbia.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD026229

PRIDE project URI

• PRIDE
  1. PXD026229
     1. Label: PRIDE project
     2. Name: Axonal and somatodendritic proteomes of dopamine neurons in the mouse brain