PXD026227

PXD026227 is an original dataset announced via ProteomeXchange.

Title	Ribosome heterogeneity in Drosophila melanogaster gonads through paralog-switching
Description	Ribosomes have long been thought of as homogeneous macromolecular machines, but recent evidence suggests they are heterogeneous and could be specialised to regulate translation. Here, we have isolated ribosomal complexes and characterised their protein content across 4 tissues of Drosophila melanogaster via TMT-MS. We find that testes and ovaries contain the most heterogeneous ribosome populations, which occurs through a combination of ribosomal protein paralog-enrichment and ribosomal protein paralog-switching. We have also solved structures of ribosomes isolated from tissues by cryo-EM, revealing differences in precise ribosomal arrangement for testis and ovary 80S ribosomes. Differences in the amino acid composition of paralog pairs and their localisation on the ribosome exterior indicate paralog-switching could alter the ribosome surface, enabling different proteins to regulate translation. One testis-specific paralog-switching pair is also found in humans, suggesting this is a conserved site of ribosome heterogeneity. Overall, this work allows us to propose that mRNA translation might be regulated in the gonads through ribosome heterogeneity, providing a potential means of ribosome specialisation.
HostingRepository	PRIDE
AnnounceDate	2021-07-23
AnnouncementXML	Submission_2021-07-22_22:57:20.930.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Karl Norris
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-24 07:16:01	ID requested
1	2021-07-22 10:31:26	announced
⏵ 2	2021-07-22 22:57:21	announced	2021-07-23: Updated project metadata.

10.1093/NAR/GKAB606;

Hopes T, Norris K, Agapiou M, McCarthy CGP, Lewis PA, O'Connell MJ, Fontana J, Aspden JL, Ribosome heterogeneity in Drosophila melanogaster gonads through paralog-switching. Nucleic Acids Res, 50(4):2240-2257(2022) [pubmed]

submitter keyword: gonads, testis,TMT-MS, ribosome, paralog, Drosophila melanogaster, ovary

Julie L Aspden
contact affiliation	School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, LS2 9JT, UK.
contact email	J.Aspden@leeds.ac.uk
lab head
Karl Norris
contact affiliation	University of Leeds
contact email	fbskn@leeds.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD026227

PRIDE project URI

• PRIDE
  1. PXD026227
     1. Label: PRIDE project
     2. Name: Ribosome heterogeneity in Drosophila melanogaster gonads through paralog-switching