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PXD026218

PXD026218 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleExoproteomic analysis of two MLST clade 2 strains of Clostridioides difficile from Latin America
DescriptionClostridioides difficile BI/NAP1/ribotype 027 is an epidemic hypervirulent strain found worldwide, including in Latin America. We examined the genomes and exoproteomes of two multilocus sequence type (MLST) clade 2 C. difficile strains considered hypervirulent: ICC-45 (ribotype SLO231/UK[CE]821), isolated in Brazil, and NAP1/027/ST01 (LIBA5756), isolated during a 2010 outbreak in Costa Rica. C. difficile isolates were cultured and extracellular proteins were analyzed using high-performance liquid chromatography-tandem mass spectrometry. Genomic analysis revealed that these isolates shared most of the gene composition. Only 83 and 290 NAP1/027 genes were considered singletons in ICC-45 and NAP1/027, respectively. Exoproteome analysis revealed 197 proteins, of which 192 were similar in both strains. Only five proteins were exclusive to the ICC-45 strain. These proteins were involved with catalytic and binding functions and indirectly interacted with proteins related to pathogenicity. Most proteins, including TcdA, TcdB, flagellin subunit, and cell surface protein, were overrepresented in the ICC-45 strain; 14 proteins, including mature S-layer protein, were present in higher proportions in LIBA5756. These data show close similarity between the genome and proteins in the supernatant of two strains with hypervirulent features isolated in Latin America and underscore the importance of epidemiological surveillance of the transmission and emergence of new strains.
HostingRepositoryPRIDE
AnnounceDate2021-07-01
AnnouncementXMLSubmission_2021-07-01_03:59:53.095.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGlauber Wagner
SpeciesList scientific name: Clostridioides difficile str. A-027; NCBI TaxID: 1155748;
ModificationListiodoacetamide derivatized residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-05-24 03:47:14ID requested
12021-07-01 03:59:53announced
Publication List
de Melo Pac, í, fico D, Costa CL, Moura H, Barr JR, Maia GA, Filho VB, Moreira RS, Wagner G, Domingues RMCP, Quesada-G, ó, mez C, de Oliveira Ferreira E, de Castro Brito GA, Exoproteomic analysis of two MLST clade 2 strains of Clostridioides difficile from Latin America reveal close similarities. Sci Rep, 11(1):13273(2021) [pubmed]
Keyword List
submitter keyword: Proteomics, Clostridioides difficile, rybotype, mass spectrometry, Latin America
Contact List
Gerly Brito
contact affiliationDepartment of Morphology, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceara Brazil
contact emailgerlybrito@hotmail.com
lab head
Glauber Wagner
contact affiliationFederal Univesity of Santa Catarina
contact emailglauber.wagner@ufsc.br
dataset submitter
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