PXD026216

PXD026216 is an original dataset announced via ProteomeXchange.

Title	p97/VCP as a therapy target for developing drugs against human coronavirus
Description	Understanding how human coronavirus dysregulate host proteome during infection in human cells will identify general pathways that are common to coronavirus infection. NMS-873 is an allosteric p97/VCP ATPase inhibitor and was show to have antiviral effect in multiple viruses including SARS-CoV2. We first demonstrated that genetic knock down of p97 reduced HCoV-229E, HCoV-OC43 infection and secretion. To investigate how p97/VCP assists virus infection, we used unbiased quantitative proteomics to compare dysregulated proteomes caused by HCoV-229E, HCoV-OC43 and SARS-CoV2 infection. We then compared dysregulated proteomes after HCoV-229E and HCoV-OC43 infection with and without p97 knockdown. Moreover, we elucidated the impact of p97 on different stages of viral life cycle using two potent p97 inhibitors, CB-5083 and NMS-873 and demonstrate their can block HCoV replication. Together, our data provide insights to repurpose potential cancer drugs that target the essential host protein p97/VCP.
HostingRepository	PRIDE
AnnounceDate	2021-11-03
AnnouncementXML	Submission_2021-11-03_03:45:18.576.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD026216
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Shan Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-24 03:47:08	ID requested
⏵ 1	2021-11-03 03:45:18	announced

Dataset with its publication pending

ProteomeXchange project tag: Covid-19, Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: p97, cornoavirus, proteomic

Tsui-Fen Chou
contact affiliation	BBE, Caltech
contact email	tfchou@caltech.edu
lab head
Shan Li
contact affiliation	Caltech
contact email	lflshan@caltech.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026216
     1. Label: PRIDE project
     2. Name: p97/VCP as a therapy target for developing drugs against human coronavirus