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PXD026208

PXD026208 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSS18 regulates pluripotent-somatic transition through phase separation
DescriptionThe transition from pluripotent to somatic states marks a critical event in mammalian development, but remains largely unresolved. Here we report the identification of SS18 as a regulator for pluripotent to somatic transition or PST by CRISPR-based whole genome screens. Mechanistically, SS18 forms microscopic condensates in nuclei through a C-terminal intrinsically disordered region (IDR) rich in tyrosine, which, once mutated, no longer form condensates nor rescue SS18-/- defect in PST. Yet, the IDR alone is not sufficient to rescue the defect even though it can form condensates indistinguishable from the wild type protein. We further show that its N-terminal 70aa is required for PST by interacting with BAF complex, and remains functional even swapped onto unrelated IDRs or even an artificial 24 tyrosine polypeptide. Finally, we show that SS18 mediates BAF assembly through phase separation to regulate PST. These studies suggest that SS18 plays a role in the pluripotent to somatic interface and undergoes liquid-liquid phase separation through a unique tyrosine-based mechanism.
HostingRepositoryiProX
AnnounceDate2021-05-23
AnnouncementXMLSubmission_2023-02-06_19:24:27.183.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterZiwei Zhai
SpeciesList scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-05-23 18:57:01ID requested
12021-05-23 18:59:43announced
22023-02-06 19:24:28announced2023-02-07: Update publication information.
Publication List
Kuang J, Zhai Z, Li P, Shi R, Guo W, Yao Y, Guo J, Zhao G, He J, Xu S, Wu C, Yu S, Zhou C, Wu L, Qin Y, Cai B, Li W, Wu Z, Li X, Chu S, Yang T, Wang B, Cao S, Li D, Zhang X, Chen J, Liu J, Pei D, SS18 regulates pluripotent-somatic transition through phase separation. Nat Commun, 12(1):4090(2021) [pubmed]
Keyword List
submitter keyword: SS18, BAF complex, phase separation, mouse embryonic stem cells, cell fate determination
Contact List
Duanqing Pei
contact affiliationWestlake University
contact emailpeiduanqing@westlake.edu.cn
lab head
Ziwei Zhai
contact affiliationGuangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
contact emailzwzhai@outlook.com
dataset submitter
Full Dataset Link List
iProX dataset URI