PXD026207

PXD026207 is an original dataset announced via ProteomeXchange.

Title	Extensive Mitochondrial Proteome Disturbance Lie in the Early Stage of Acute Myocardial Ischemia
Description	Background： Mitochondria malfunction leads to myocardial energy metabolism remodeling during myocardial ischemia. However, the mitochondrial proteome profile during this period has not been clarified. Methods：Acute myocardial ischemia (MI) model was established by high ligation of left anterior descending (LAD) coronary artery in 8-week-old C57BL/6N mice. After 15 min of ligation, the animals were sacrificed and hearts were collected. Myocardium ultrastructure was observed by transmission electron microscope (TEM). The cardiac mitochondrial proteome profile was analyzed via liquid chromatography-tandem mass spectrometry (LC-MS/MS) approach and bioinformatics analysis. Results：Observation of TEM showed the mitochondria outer membrane was dissolved, inner membrane (cristae) was corrupted and disappeared extensively in the MI group. The MMP was decreased in the MI group compared with the Sham (p < 0.05). More than 1,700 mitochondrial proteins were screened by LC-MS/MS analysis, and 119 differentially expressed proteins were identified in the MI group (p < 0.05). 27 of the differential proteins were up-regulated and 16 were down-regulated, and the other 76 proteins showed differences between “with or without”. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analysis showed that endopeptidase activity regulation, mitochondrial inner membrane, oxidative phosphorylation, hypoxia-inducible factor-1 (HIF-1) signal pathway, pentose phosphate pathway, and PPAR signal pathway were involved in the pathophysiological process in the early stage of acute myocardial ischemia. Conclusion：Extensive and significant changes of mitochondrial proteins along with mitochondria microstructure damages arise in the early stage of acute MI. Series of the proteins are crucial in the pathways of mitochondrial dysfunction, positive regulation of mitochondrion autophagy in respiratory, collagen-containing extracellular matrix, and metabolism. Further studies are needed to clarify the roles and details of these proteins in acute MI injury.
HostingRepository	iProX
AnnounceDate	2021-05-21
AnnouncementXML	Submission_2023-02-02_19:05:50.235.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jun he
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-23 18:45:02	ID requested
1	2021-05-23 18:45:41	announced
⏵ 2	2023-02-02 19:05:51	announced	2023-02-03: Update publication information.

Wang J, He J, Fan Y, Xu F, Liu Q, He R, Yan R, Extensive mitochondrial proteome disturbance occurs during the early stages of acute myocardial ischemia. Exp Ther Med, 23(1):85(2022) [pubmed]

submitter keyword: acute myocardial ischemia, mitochondria, proteome, myocardial remodeling, liquid chromatography-tandem mass spectrometry

Jun he
contact affiliation	Department of Cardiovascular Internal Medicine, General Hospital of Ningxia Medical University
contact email	junhe@nyfy.com.cn
lab head
Jun he
contact affiliation	Department of Cardiovascular Internal Medicine, General Hospital of Ningxia Medical University
contact email	junhe@nyfy.com.cn
dataset submitter

iProX dataset URI