PXD026206

PXD026206 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Datasets for FLASHIda: Intelligent data acquisition for top-down proteomics that doubles proteoform identification count
Description	Four sets top-down MS single runs from E.coli (K12 MS1655 i) lysate with FLASHIda and standard acquisition. Two sets using FLASHIda acquisition and two using standard acquisition for intact proteins (Intact Protein mode) on a Thermo Scientific Orbitrap Eclipse. Two sets from each acquisition method have different RT gradients of 30 minutes and 90 minutes. Lastly, each set contains technical triplicates. Thus we have three 90 minute gradient single runs with FLASHIda acquisition (FI90 datasets), three 30 minutes FLASHIda (FI30 datasets), three 90 minute standard (ST90 datasets), and three 30 minute standard (ST30 datasets) runs. ST90: Eclip_1476_MB.mzML, Eclip_1479_MB.mzML, Eclip_1480_MB ST30: Eclip_1477_MB.mzML, Eclip_1483_MB.mzML, Eclip_1485_MB FI90: Eclip_1481_MB.mzML, Eclip_1482_MB.mzML, Eclip_1486_MB FI30: Eclip_1478_MB_20210423200930.mzML, Eclip_1484_MB.raw, Eclip_1487_MB
HostingRepository	MassIVE
AnnounceDate	2021-06-27
AnnouncementXML	Submission_2021-06-27_10:25:16.383.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	kyowonjeong
SpeciesList	scientific name: E.coli K12 MG1655 i;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-22 13:13:38	ID requested
⏵ 1	2021-06-27 10:25:16	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: top-down proteomics, data acquisition, FLASHDeconv

submitter keyword: top-down proteomics, data acquisition, FLASHDeconv

Contact List

Oliver Kohlbacher
contact affiliation	Tuebingen University
contact email	oliver.kohlbacher@uni-tuebingen.de
lab head
kyowonjeong
contact affiliation	Tuebingen University
contact email	kyowonjeong@gmail.com
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000087484/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000087484/