PXD026200

PXD026200 is an original dataset announced via ProteomeXchange.

Title	The proteome of developing barley anthers during meiotic prophase I
Description	In the current project, we aimed to discover how the barley anther proteome changes, both quantitatively and qualitatively, during meiotic prophase I when the important process of homologous recombination is taking place. Taking advantage of a recently published barley anther transcriptomic dataset (BAnTr) and a newly developed sensitive mass spectrometry-based approach to analyse barley anther proteome, we have conducted high-resolution mass spectrometry analysis of barley anthers, collected at 6 time points and representing their development from pre-meiosis to metaphase. Each time point was carefully staged using immunocytology. We identified >6400 proteins, including 82 known and putative meiotic proteins, and were able to quantify dynamic variation in the proteome during different stages of barley anther development.
HostingRepository	PRIDE
AnnounceDate	2022-05-27
AnnouncementXML	Submission_2022-05-27_05:03:33.572.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Runxuan Zhang
SpeciesList	scientific name: Hordeum vulgare (Barley); NCBI TaxID: 4513;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-21 05:48:50	ID requested
⏵ 1	2022-05-27 05:03:34	announced

Lewandowska D, Orr J, Schreiber M, Colas I, Ramsay L, Zhang R, Waugh R, The proteome of developing barley anthers during meiotic prophase I. J Exp Bot, 73(5):1464-1482(2022) [pubmed]

submitter keyword: barley, anther, meiosis

Robbie Waugh
contact affiliation	The James Hutton Institute, Dundee, UK
contact email	robbie.waugh@hutton.ac.uk
lab head
Runxuan Zhang
contact affiliation	The James Hutton Institute
contact email	runxuan.zhang@hutton.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD026200

PRIDE project URI

• PRIDE
  1. PXD026200
     1. Label: PRIDE project
     2. Name: The proteome of developing barley anthers during meiotic prophase I