PXD026192

PXD026192 is an original dataset announced via ProteomeXchange.

Title	Metaproteomic analysis of nine coral species with and without Stony Coral Tissue Loss Disease (SCTLD)
Description	Florida’s coral reefs are currently experiencing a multi-year disease-related mortality event, that has resulted in massive die-offs in multiple coral species. Approximately 21 species of coral, including both Endangered Species Act-listed and the primary reef-building species, have displayed tissue loss lesions which often result in whole colony mortality [Stony Coral Tissue Loss Disease (SCTLD)]. Determining the causative agent(s) of coral disease relies on a multidisciplinary approach since the causation may be a combination of abiotic, microbial or viral agents. Metaproteomics was used to survey changes in the molecular landscape in the coral holobiont with the goal of providing useful information not only in diagnosis, but for prediction and prognosis. Specifically, in the case of SCTLD, defining molecular changes in the coral holobiont will help define disease progression and aid in identifying the causative agent by clearly defining traits of disease progression shared across affected species. Using samples from nine coral species (46 samples total; those appearing healthy, n = 23, and diseased, n = 23), analysis of the coral and its associated microbiome were performed using bottom-up proteomics. Ongoing analysis (including improving coral holobiont genome-based search space) will demonstrate the utility of this approach and help define improved future experiments.
HostingRepository	PRIDE
AnnounceDate	2021-05-21
AnnouncementXML	Submission_2021-05-21_05:19:36.950.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD026192
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Benjamin Neely
SpeciesList	scientific name: Colpophyllia natans; NCBI TaxID: 242718; scientific name: Dichocoenia stokesii; NCBI TaxID: 1499105; scientific name: Pseudodiploria clivosa; NCBI TaxID: 1917265; scientific name: Montastraea cavernosa; NCBI TaxID: 63558; scientific name: Pseudodiploria strigosa; NCBI TaxID: 1428006; scientific name: Orbicella annularis; NCBI TaxID: 48500; scientific name: Orbicella faveolata; NCBI TaxID: 48498; scientific name: Diploria labyrinthiformis; NCBI TaxID: 242715; scientific name: Meandrina meandrites; NCBI TaxID: 51056;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-21 04:16:29	ID requested
⏵ 1	2021-05-21 05:19:37	announced

Dataset with its publication pending

submitter keyword: metaproteomics, corals, SCTLD

Benjamin A. Neely, Cheryl M. Woodley, Michael G. Janech
contact affiliation	Hollings Marine Laboratory
contact email	benjamin.neely@nist.gov
lab head
Benjamin Neely
contact affiliation	National Institute of Standards and Technology
contact email	benjamin.neely@nist.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026192
     1. Label: PRIDE project
     2. Name: Metaproteomic analysis of nine coral species with and without Stony Coral Tissue Loss Disease (SCTLD)