PXD026183

PXD026183 is an original dataset announced via ProteomeXchange.

Title	STN7 kinase-dependent Light-harvesting complex (LHC) protein phosphorylations in the moss Physcomitrium (Physcomitrella) patens
Description	Photosystem (PS) I and PSII enclose the reaction center subunits and cofactors responsible for the conversion of sunlight into chemical energy. In the thylakoid membrane of oxygenic photosynthetic organisms, PSII splits the water molecules and donates electrons to the electron transfer chain, and further via PSI to form NADPH. Proteins of the light-harvesting complexes (LHC) I and II are mainly associated with PSI and PSII, respectively, but also provide dynamics for the adjustments of the absorption cross-section of PSII and PSI upon changing light conditions. LHCII proteins N-terminal phosphorylation affects their interaction either with PSI or with PSII, and is a molecular mechanism present from green algae and early land plants to higher plants. The kinase STN7 is responsible for N-terminal phosphorylation of the LHCII proteins in algae and flowering plants model species, but the specific targets and role of STN7-dependent reversible phosphorylation in formation of various PS-LHC complexes in evolutionarily early land plants like mosses is poorly studied. The aim of this project was to identify the phosphorylated residues of the LHCII subunits of the moss Physcomitrium (Physcomitrella) patens (hereafter: P. patens), i.e. the LHCBM proteins, and to determine which of the phosphosites are phosphorylated by the STN7 kinase. Subsequently, the goal has been to identify which of the LHCBM subunits that are part of PSI-LHCI-LHCII complex (state complex), and of the moss-specific PSI-Large complex, are N-terminally phosphorylated in the STN7-dependent fashion.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:52:23.106.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD026183
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrea Trotta
SpeciesList	scientific name: Physcomitrella patens subsp. patens (Moss); NCBI TaxID: 3218;
ModificationList	phosphorylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-21 03:50:53	ID requested
1	2022-06-24 00:13:58	announced
⏵ 2	2023-11-14 08:52:23	announced	2023-11-14: Updated project metadata.

10.6019/PXD026183;

submitter keyword: Photosystem I, N-terminal phosphorylation,Physcomitrella patens, PSI-large, Physcomitrium patens, Light-harvesting complex II

Eva-Mari Aro
contact affiliation	Molecular Plant Biology, University of Turku
contact email	evaaro@utu.fi
lab head
Andrea Trotta
contact affiliation	Institute of Biosciences and Bioresources, National Research Council of Italy, Sesto Fiorentino (FI)
contact email	andrea.trotta@utu.fi
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026183
     1. Label: PRIDE project
     2. Name: STN7 kinase-dependent Light-harvesting complex (LHC) protein phosphorylations in the moss Physcomitrium (Physcomitrella) patens