PXD026160

PXD026160 is an original dataset announced via ProteomeXchange.

Title	Data for the Blood Proteoform Atlas:DS-54:Monocyte:Purchased from STC
Description	Human biology is tightly linked to proteins, yet most measurements do not precisely determine their full sequence and post-translational modifications. Here, we present the primary structures of 30,000 unique proteoforms expressed from 1,690 human genes across 21 cell types and plasma from human blood and bone marrow compiled in the Blood Proteoform Atlas (BPA). Our results indicate that while a given protein can be expressed across multiple cell types, the proteoform functions as a more specific indicator of differentiation. These results provide a better biochemical description of protein-level biology expressed through gene transcription and translation. We demonstrate the utility of the BPA by focusing on cell- and proteoform-specific signatures within 58 liver transplant recipients having healthy graft function or undergoing acute organ rejection or dysfunction.
HostingRepository	PRIDE
AnnounceDate	2022-02-03
AnnouncementXML	Submission_2022-02-03_08:03:12.711.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Hollas
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-20 10:59:12	ID requested
⏵ 1	2022-02-03 08:03:13	announced

Melani RD, Gerbasi VR, Anderson LC, Sikora JW, Toby TK, Hutton JE, Butcher DS, Negr, ã, o F, Seckler HS, Srzenti, ć K, Fornelli L, Camarillo JM, LeDuc RD, Cesnik AJ, Lundberg E, Greer JB, Fellers RT, Robey MT, DeHart CJ, Forte E, Hendrickson CL, Abbatiello SE, Thomas PM, Kokaji AI, Levitsky J, Kelleher NL, The Blood Proteoform Atlas: A reference map of proteoforms in human hematopoietic cells. Science, 375(6579):411-418(2022) [pubmed]

submitter keyword: BPA, top-down proteomics, blood cell types, plasma

Neil L. Kelleher
contact affiliation	Departments of Chemical and Biological Engineering, Chemistry, and Molecular Biosciences, the Chemistry of Life Processes Institute, and the Proteomics Center of Excellence, Northwestern University, Evanston, Illinois 60208, United States
contact email	n-kelleher@NORTHWESTERN.EDU
lab head
Michael Hollas
contact affiliation	Northwestern University
contact email	michael.hollas@northwestern.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD026160

PRIDE project URI

• PRIDE
  1. PXD026160
     1. Label: PRIDE project
     2. Name: Data for the Blood Proteoform Atlas:DS-54:Monocyte:Purchased from STC