PXD026118

PXD026118 is an original dataset announced via ProteomeXchange.

Title	Integrative analysis of multi-omics datasets for Synechocystis sp. PCC 6803 identifies novel elements in the salt acclimation process of cyanobacteria
Description	The salt acclimation process of the euryhaline model cyanobacterium Synechocystis sp. PCC 6803 was analyzed by combining transcriptomic, proteomic and metabolomic methods. The comparison of salt-induced proteome and transcriptome changes revealed that most stably up-regulated proteins also showed elevated mRNA levels. The Pearson correlation coefficient for salt-induced abundance changes of 1749 transcript/protein pairs was r = 0.58. In addition to the rapid and stable upregulation of compatible solute biochemistry, a dynamic reorganization of the transcriptome occurred during the first hours after salt shock, which probably involves the action of small regulatory RNAs. Based on these data, an extended salt stimulon can be defined comprising many proteins directly or indirectly related to compatible solute metabolism, ion and water movements as well as a defined set of small regulatory RNAs. Our comprehensive data set provides the basis for future attempts to engineer cyanobacterial salt tolerance and to search for processes regulating this important environmental acclimation process.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_04:42:06.765.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD026118
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Stefan Mikkat
SpeciesList	scientific name: Synechocystis sp. PCC 6803 substr. Kazusa; NCBI TaxID: 1111708;
ModificationList	iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-20 03:31:21	ID requested
⏵ 1	2022-02-17 04:42:07	announced

Kl, ä, hn S, Mikkat S, Riediger M, Georg J, Hess WR, Hagemann M, Integrative analysis of the salt stress response in cyanobacteria. Biol Direct, 16(1):26(2021) [pubmed]

submitter keyword: Synechocystis sp. PCC 6803, HDMSE, Label-free quantification, Salt acclimation, Transcriptome-Proteome correlation

Martin Hagemann
contact affiliation	Department of Plant Physiology, University of Rostock, Einsteinstr. 3, D-18059 Rostock, Germany
contact email	martin.hagemann@uni-rostock.de
lab head
Stefan Mikkat
contact affiliation	Core Facility Proteome Analysis, University Medicine Rostock, Schillingallee 69, 18057 Rostock
contact email	stefan.mikkat@med.uni-rostock.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026118
     1. Label: PRIDE project
     2. Name: Integrative analysis of multi-omics datasets for Synechocystis sp. PCC 6803 identifies novel elements in the salt acclimation process of cyanobacteria