PXD026117

PXD026117 is an original dataset announced via ProteomeXchange.

Title	LC-MS analysis of in vitro SUMOylated SRFR1 protein.
Description	FLAG-SRFR1 was expressed in reconstituted E. coli system (Okada el al 2009) in the presence of SUMO1 T91R SUMOylation-competent (containing diglycine, GG) or -deficient (diglycine replaced by dialanine, AA) isoforms, SCE1a, and SAE1/2. After induction of expressions, total extracts were probed with anti-FLAG antibodies. A slower migrating band than FLAG-SRFR1 was detected in the presence of only GG, but not AA forms of co- expressed SUMO1. We then subjected the slower migrating protein bands of SRFR1 expressed with SUMO1 T91R for trypsin digestion and LC-MS/MS analysis. SUMO1, but not SUMO2 or SUMO3 footprints on selective lysine residues of SRFR1 was detected in the identified peptides. Remarkably, lysine residue present in the previously predicted LK325EE SUMOylation motif was identified with SUMO1-modifications. In addition, the K229 residue of SRFR1 was also covalently modified by SUMO1 even though conserved features of a SUMOylation motif was absent for this lysine.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_08:52:49.231.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD026117
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kishor Ingole
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-20 03:31:21	ID requested
⏵ 1	2022-02-17 08:52:50	announced

Kasera M, Ingole KD, Rampuria S, Walia Y, Gassmann W, Bhattacharjee S, . Front Cell Dev Biol, 9():680760(2021) [pubmed]

submitter keyword: Arabidopsis, SRFR1, SUMOylation, LC-MS/MS

Dr. Saikat Bhattacharjee
contact affiliation	Regional Centre for Biotechnology, Faridabad, Haryana, India
contact email	saikat@rcb.res.in
lab head
Kishor Ingole
contact affiliation	Postdoctoral Researcher
contact email	kishor.ingole@rcb.res.in
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026117
     1. Label: PRIDE project
     2. Name: LC-MS analysis of in vitro SUMOylated SRFR1 protein.