PXD026105

PXD026105 is an original dataset announced via ProteomeXchange.

Title	Comparative analysis of stalked and acorn barnacle adhesive proteomesv
Description	Barnacles interest the scientific community for multiple reasons: their unique evolutionary trajectory, vast diversity, and economic impact as a harvested food source and also as one of the most prolific macroscopic hard biofouling organisms. A common, yet novel, trait among barnacles is adhesion, which has enabled a sessile adult existence and global colonization of the oceans. Barnacle adhesive is primarily composed of proteins, but knowledge of how the adhesive proteome varies across the tree of life is unknown due to a lack of genomic information. Here, we supplement previous mass spectrometry analyses of barnacle adhesive with recently sequenced genomes to compare the adhesive proteomes of Pollicipes pollicipes (Pedunculata) and Amphibalanus amphitrite (Sessilia). Although both species contain the same broad protein categories, we detail differences that exist between these species. The barnacle-unique cement proteins show the greatest difference between species, although these differences are diminished when amino acid composition and glycosylation potential are considered. By performing an in-depth comparison of the adhesive proteomes of these distantly related barnacle species, we show their similarities and provide a roadmap for future studies examining sequence specific differences to identify the proteins responsible for functional differences across the barnacle tree of life.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_08:57:33.909.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Janna Schultzhaus
SpeciesList	scientific name: Amphibalanus amphitrite; NCBI TaxID: 1232801;
ModificationList	acetylated residue; deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-19 22:45:45	ID requested
⏵ 1	2022-02-17 08:57:34	announced

Schultzhaus JN, Hervey WJ, Taitt CR, So CR, Leary DH, Wahl KJ, Spillmann CM, Comparative analysis of stalked and acorn barnacle adhesive proteomes. Open Biol, 11(8):210142(2021) [pubmed]

submitter keyword: Pedunculata, Sessilia, Pollicipes pollicipes, Amphibalanus amphitrite, cement proteins, mass spectrometry

Judson Hervey
contact affiliation	NRL
contact email	judson.hervey@nrl.navy.mil
lab head
Janna Schultzhaus
contact affiliation	NRL
contact email	jannaniehaus@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026105
     1. Label: PRIDE project
     2. Name: Comparative analysis of stalked and acorn barnacle adhesive proteomesv