PXD026101 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The Prostate Cancer mTOR Chromatin-Bound Interactome |
Description | Growing studies support a direct role for nuclear mTOR in gene regulation and chromatin structure. Still, the scarcity of known chromatin-bound mTOR partners limits our understanding of how nuclear mTOR controls transcription. Herein, we comprehensively mapped the mTOR chromatin-bound interactome in four cellular models of prostate cancer (PCa) identifying a conserved 67-protein interaction network enriched for epigenetic and transcription factors as well as SUMOylation machinery in both androgen-dependent and -independent cells. Notably, SUMO2/3 and nuclear pore protein NUP210 are among the strongest interactors while the androgen receptor (AR) is the dominant androgen-inducible mTOR partner. Further investigation showed that NUP210 facilitates mTOR nuclear trafficking, that mTOR, AR and NuRD act as a functional transcriptional complex, and that androgens dictate mTOR-SUMO2/3 promoter-enhancer specificity. This work identifies a vast network of mTOR-associated nuclear complexes advocating novel molecular strategies to modulate mTOR-dependent gene regulation with evident implications for PCa and other diseases. |
HostingRepository | PRIDE |
AnnounceDate | 2022-04-01 |
AnnouncementXML | Submission_2022-04-01_01:52:14.428.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD026101 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Vincent Giguere |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-05-19 22:37:52 | ID requested | |
⏵ 1 | 2022-04-01 01:52:15 | announced | |
Publication List
Dufour CR, Scholtes C, Yan M, Chen Y, Han L, Li T, Xia H, Deng Q, Vernier M, Gigu, รจ, re V, The mTOR chromatin-bound interactome in prostate cancer. Cell Rep, 38(12):110534(2022) [pubmed] |
Keyword List
submitter keyword: Human, mTOR, ChIP-MS, RIME, Prostate cancer, LNCaP, 22Rv1, DU145, PC3, normal prostate, RWPE-1 |
Contact List
Vincent Giguere |
contact affiliation | McGill University - Rosalind & Morris Goodman Cancer Research Centre |
contact email | vincent.giguere@mcgill.ca |
lab head | |
Vincent Giguere |
contact affiliation | McGill University |
contact email | vincent.giguere@mcgill.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD026101
- Label: PRIDE project
- Name: The Prostate Cancer mTOR Chromatin-Bound Interactome