PXD026101

PXD026101 is an original dataset announced via ProteomeXchange.

Title	The Prostate Cancer mTOR Chromatin-Bound Interactome
Description	Growing studies support a direct role for nuclear mTOR in gene regulation and chromatin structure. Still, the scarcity of known chromatin-bound mTOR partners limits our understanding of how nuclear mTOR controls transcription. Herein, we comprehensively mapped the mTOR chromatin-bound interactome in four cellular models of prostate cancer (PCa) identifying a conserved 67-protein interaction network enriched for epigenetic and transcription factors as well as SUMOylation machinery in both androgen-dependent and -independent cells. Notably, SUMO2/3 and nuclear pore protein NUP210 are among the strongest interactors while the androgen receptor (AR) is the dominant androgen-inducible mTOR partner. Further investigation showed that NUP210 facilitates mTOR nuclear trafficking, that mTOR, AR and NuRD act as a functional transcriptional complex, and that androgens dictate mTOR-SUMO2/3 promoter-enhancer specificity. This work identifies a vast network of mTOR-associated nuclear complexes advocating novel molecular strategies to modulate mTOR-dependent gene regulation with evident implications for PCa and other diseases.
HostingRepository	PRIDE
AnnounceDate	2022-04-01
AnnouncementXML	Submission_2022-04-01_01:52:14.428.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD026101
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Vincent Giguere
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-19 22:37:52	ID requested
⏵ 1	2022-04-01 01:52:15	announced

Dufour CR, Scholtes C, Yan M, Chen Y, Han L, Li T, Xia H, Deng Q, Vernier M, Gigu, è, re V, The mTOR chromatin-bound interactome in prostate cancer. Cell Rep, 38(12):110534(2022) [pubmed]

submitter keyword: Human, mTOR, ChIP-MS, RIME, Prostate cancer, LNCaP, 22Rv1, DU145, PC3, normal prostate, RWPE-1

Vincent Giguere
contact affiliation	McGill University - Rosalind & Morris Goodman Cancer Research Centre
contact email	vincent.giguere@mcgill.ca
lab head
Vincent Giguere
contact affiliation	McGill University
contact email	vincent.giguere@mcgill.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026101
     1. Label: PRIDE project
     2. Name: The Prostate Cancer mTOR Chromatin-Bound Interactome