⮝ Full datasets listing

PXD026093

PXD026093 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBeyond self-resistance: ABCF ATPase LmrC is a signal-transducing component of an antibiotic-driven signaling cascade hastening the onset of lincomycin biosynthesis
DescriptionIn natural environments, antibiotics are an important instrument of inter-species competition. At subinhibitory concentrations, they act as cues or signals inducing antibiotic production: however, our knowledge of well-documented antibiotic-based sensing systems is limited. Here, for the soil actinobacterium Streptomyces lincolnensis we describe a fundamentally new ribosome-mediated signaling cascade that accelerates the onset of lincomycin production in response to an external ribosome-targeting antibiotic to synchronize the antibiotic production within the population. The entire cascade is encoded within the lincomycin biosynthetic gene cluster (BGC) and besides the transcriptional regulator, LmbU it consists of three lincomycin resistance proteins: a lincomycin transporter, LmrA, a 23S rRNA methyltransferase, LmrB, both conferring a high resistance, and an ABCF ATPase LmrC that confers only moderate resistance but is indispensable for the antibiotic-induced signal transduction. Specifically, the antibiotic sensing occurs via a ribosome-mediated attenuation, which activates LmrC production in response to lincosamide, streptogramin A, or pleuromutilin antibiotics. Then, the ribosome-operating LmrC ATPase activity triggers the transcription of lmbU and consequently the expression of lincomycin BGC. Finally, the production of LmrC is downregulated by LmrA and LmrB which reduces the amount of the ribosome-bound antibiotic and thus fine-tune the cascade. We propose that analogous ABCF-mediated signaling systems are relatively common because many BGCs for ribosome-targeting antibiotics encode an ABCF-protein accompanied by additional resistance protein(s) and transcriptional regulators. Moreover, we revealed that three of eight co-produced ABCF proteins of S. lincolnensis are clindamycin-responsive thus the ABCF-mediated antibiotic signaling might be generally utilized tool of chemical communication.
HostingRepositoryPRIDE
AnnounceDate2021-08-18
AnnouncementXMLSubmission_2021-08-18_06:18:40.328.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD026093
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterKarel Harant
SpeciesList scientific name: Streptomyces lincolnensis; NCBI TaxID: 1915;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-05-19 07:01:00ID requested
12021-08-18 06:18:40announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: resistance, Streptomyces lincolnensis, lincomycin, LmrC
Contact List
Gabriela Balíková Novotná, PhD.
contact affiliationHead of the Antibiotic Resistance Research group Laboratory for Biology of Secondary Metabolism Institute of Microbiology BIOCEV Průmyslová 959 252 42 Vestec Czech Republic
contact emailgnovotna@biomed.cas.cz
lab head
Karel Harant
contact affiliationCharles University
contact emailharant@natur.cuni.cz
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD026093
PRIDE project URI
Repository Record List
[ + ]