PXD026092

PXD026092 is an original dataset announced via ProteomeXchange.

Title	Revisiting Ehrlichia ruminantium Replication Cycle Using Proteomics : The Host and the Bacterium Perspectives
Description	The Rickettsiales Ehrlichia ruminantium (ER), the causal agent of the fatal tick-borne disease Heartwater, induces severe damage to the vascular endothelium in ruminants. Nevertheless, E. ruminantium-induced pathobiology remains largely unknown. Our work paves the way for understanding this phenomenon by using quantitative proteomic analyses (2D-DIGE-MS/MS, 1DE-nanoLC-MS/MS and biotin-nanoUPLC-MS/MS) of host bovine aorta endothelial cells (BAE) during the in vitro bacterium intracellular replication cycle. We detect 265 bacterial proteins (including virulence factors), at all time-points of the E. ruminantium replication cycle, highlighting a dynamic bacterium–host interaction. We show that E. ruminantium infection modulates the expression of 433 host proteins: 98 being over-expressed, 161 under-expressed, 140 detected only in infected BAE cells and 34 exclusively detected in non-infected cells. Cystoscape integrated data analysis shows that these proteins lead to major changes in host cell immune responses, host cell metabolism and vesicle trafficking, with a clear involvement of inflammation-related proteins in this process. Our findings led to the first model of E. ruminantium infection in host cells in vitro, and we highlight potential biomarkers of E. ruminantium infection in endothelial cells (such as ROCK1, TMEM16K, Albumin and PTPN1), which may be important to further combat Heartwater, namely by developing non-antibiotic-based strategies.
HostingRepository	PRIDE
AnnounceDate	2021-07-14
AnnouncementXML	Submission_2021-07-13_21:45:41.236.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gabriel Mazzucchelli
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Ehrlichia ruminantium str. Gardel; NCBI TaxID: 302409;
ModificationList	iodoacetamide derivatized residue
Instrument	Synapt MS; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-19 07:00:15	ID requested
⏵ 1	2021-07-13 21:45:42	announced

Marcelino I, Holzmuller P, Coelho A, Mazzucchelli G, Fernandez B, Vachi, é, ry N, Replication Cycle Using Proteomics: The Host and the Bacterium Perspectives. Microorganisms, 9(6):(2021) [pubmed]

submitter keyword: Ehrlichia ruminantium

endothelial cells

host response

immunomodulation

bacterial life cycle

differential protein expression

infection biomarkers

virulence factors

Nathalie VACHIERY
contact affiliation	ASTRE, Univ Montpellier, CIRAD, INRA, Montpellier, France + CIRAD, UMR ASTRE, F-34398, Montpellier, France
contact email	nathalie.vachiery@cirad.fr
lab head
Gabriel Mazzucchelli
contact affiliation	Mass Spectrometry Laboratory, MolSys Research Unit, University of Liege, Liege 4000, Belgium
contact email	gabriel.mazzucchelli@uliege.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD026092

PRIDE project URI

• PRIDE
  1. PXD026092
     1. Label: PRIDE project
     2. Name: Revisiting Ehrlichia ruminantium Replication Cycle Using Proteomics : The Host and the Bacterium Perspectives