PXD026089

PXD026089 is an original dataset announced via ProteomeXchange.

Title	Identification of trans-acting factors mediating mRNA localization
Description	Here we aimed to identify protein interacting partners of the (AU)8 motif mediating mRNA localization. For that, RNA carrying this motifs and its mutated version (ATATATATATATATAT ➝ GTACATACATGTACAT) is included in in vitro produced RNA containing 5 boxB sites, the RNA is incubated with mouse brain lysate and fished out using GRNA chromatography. Bound proteins were analysed by mass spectrometry and ELAVL2, ELAVL3 and ELAVl4 identified among the most enriched interactions of AU)8 motif.
HostingRepository	PRIDE
AnnounceDate	2022-11-15
AnnouncementXML	Submission_2022-11-15_08:32:21.185.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MarieluiseKirchner
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-19 06:46:28	ID requested
⏵ 1	2022-11-15 08:32:21	announced

Dataset with its publication pending

submitter keyword: mouse, GRNA chromatography, RNA motif, LC-MS/MS

PhilippMertins
contact affiliation	Proteomics Platform , BIH at Charite, Robert Roessle Strasse 10, 13125 Berlin
contact email	Philipp.mertins@mdc-berlin.de
lab head
MarieluiseKirchner
contact affiliation	Proteomics Platform, BIH@Charite
contact email	marieluise.kirchner@mdc-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/11/PXD026089

PRIDE project URI

• PRIDE
  1. PXD026089
     1. Label: PRIDE project
     2. Name: Identification of trans-acting factors mediating mRNA localization