PXD026072

PXD026072 is an original dataset announced via ProteomeXchange.

Title	Caught in the act: multiphoton microscopy reveals host extracellular matrix remodeling by a human cancer-avatar model
Description	: Cancer cells facilitate tumor growth by creating favorable tumor micro-environments (TME), altering homeostasis and immune response in the extracellular matrix (ECM) of surrounding tissue. A potential factor that contributes to TME generation and ECM remodeling is the cytoskeleton-associated human death-associated protein kinase 1 (DAPK1). Increased tumor cell motility and de-adhesion (thus, promoting metastasis), as well as upregulated plasminogen-signaling are shown when functionally analyzing the DAPK1 ko related proteome. However, the systematic investigation how tumor cells actively modulate the ECM at the tissue level is experimentally challenging, since animal models do not allow direct experimental access while artificial in vitro scaffolds cannot simulate the entire complexity of tissue systems. Here, we used the chorioallantoic membrane (CAM) assay as a natural, collagen-rich tissue model in combination with an all-optical experimental access by multiphoton microscopy (MPM) in order to study the ECM remodeling potential of colorectal tumor cells with and without DAPK1 in situ and even in vivo. This approach demonstrates the suitability of the CAM assay in combination with multiphoton microscopy for studying collagen remodeling during tumor growth. Our results indicate the high ECM remodeling potential of DAPK1 ko tumor cells at the tissue level and support our findings from proteomics.
HostingRepository	PRIDE
AnnounceDate	2022-06-09
AnnouncementXML	Submission_2022-06-09_05:14:00.662.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD026072
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Karel Harant
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	methylthiolated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-18 06:23:41	ID requested
⏵ 1	2022-06-09 05:14:04	announced

Kunze P, Kreiss L, Novosadov, á V, Roehe AV, Steinmann S, Prochazka J, Geppert CI, Hartmann A, Sch, ü, rmann S, Friedrich O, Schneider-Stock R, Multiphoton Microscopy Reveals DAPK1-Dependent Extracellular Matrix Remodeling in a Chorioallantoic Membrane (CAM) Model. Cancers (Basel), 14(10):(2022) [pubmed]

submitter keyword: CRC, ECM remodeling, collagen, multiphoton microscopy, CAM

Regine Schneider-Stock
contact affiliation	Experimental Tumor Pathology, Institute of Pathology, University Hospital Erlangen, Friedrich‐Alexander University Erlangen‐Nürnberg (FAU), Erlangen 91054, Germany
contact email	regine.schneider-stock@uk-erlangen.de
lab head
Karel Harant
contact affiliation	Charles University
contact email	harant@natur.cuni.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026072
     1. Label: PRIDE project
     2. Name: Caught in the act: multiphoton microscopy reveals host extracellular matrix remodeling by a human cancer-avatar model