<<< Full experiment listing

PXD026062

PXD026062 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of AcpP-MukB binding sites using cross-linking mass spectrometry
DescriptionStructural Maintenance of Chromosomes (SMC) complexes contribute ubiquitously to chromosome organization-segregation. SMC proteins have a conserved architecture, with a dimerization hinge and an ATPase head domain separated by a long antiparallel intramolecular coiled-coil. Dimeric SMC proteins interact with essential accessory proteins, kleisins that bridge the two subunits of an SMC dimer, and HAWK/KITE accessory proteins that interact with kleisins. The ATPase activity of the Escherichia coli SMC protein, MukB, is essential for in vivo function and is regulated by interactions with its dimeric kleisin, MukF, and KITE, MukE. Here we demonstrate that, in addition, MukB interacts with Acyl Carrier Protein (AcpP) that has essential functions in fatty acid synthesis. We characterize the AcpP interaction site at the joint of the MukB coiled-coil and show that the interaction is essential for MukB ATPase and for MukBEF function in vivo. Therefore, AcpP is an essential co-factor for MukBEF action in chromosome organization-segregation
HostingRepositoryPRIDE
AnnounceDate2021-11-25
AnnouncementXMLSubmission_2021-11-25_09:01:44.128.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMarjorie Fournier
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListacetylated residue; monohydroxylated residue; iodoacetic acid derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-05-17 11:48:32ID requested
12021-11-25 09:01:44announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Structural Maintenance of Chromosomes, MukB, AcpP, cross-linking mass spectrometry
Contact List
David Sherratt
contact affiliationDepartment of Biochemistry, University of Oxford
contact emaildavid.sherratt@bioch.ox.ac.uk
lab head
Marjorie Fournier
contact affiliationUniversity of Oxford
contact emailmarjorie.fournier@bioch.ox.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
PRIDE project URI
Repository Record List
[ + ]