PXD026058

PXD026058 is an original dataset announced via ProteomeXchange.

Title	Exposure of Apis mellifera to anti-Varroa destructor formic acid treatment induces significant proteomic alterations.
Description	The parasitic mite Varroa destructor is a major threat to the health and productivity of Apis mellifera hives and induces its effect by feeding on the fat body of larvae/pupae and transmitting viruses. Control of Varroa populations can be attempted using a variety of treatments and the effect of one of these (the formic acid containing - Mite Away Quick (MAQ) strips) on the proteome of A. mellifera was assessed in this work. Samples of A. mellifera were isolated from hives one week prior to MAQ treatment, during the week of treatment and for two weeks after end of treatment, proteins were extracted and analysed by label free quantitative proteomics. The results indicated that samples isolated during the week of treatment showed increased abundance of a range of cuticular proteins (+ 2.65 fold to + 6.64 fold) and decreased abundance of proteins that deal with xenobiotics (Cytochrome P450 subunits -11.38 fold to -2.16 fold) Interestingly some proteins associated with the oxidative phosphorylation pathway were increased in abundance (Cox5a and Cox5b) but others such as (Coxfa4) were decreased. The results presented here reveal that application of MAQ strips caused a dramatic disruption to the proteome of A. mellifera but the effect is transient and that by two weeks after the end of treatment the proteome has returned to resemble that of the untreated control. While MAQ strips are effective in reducing Varroa populations, the results presented here indicate they can adversely affect the proteome of A. mellifera and may contribute to the elevated stress in hives previously affected by Varroa parasitisation.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:16:30.667.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rachel Ward
SpeciesList	scientific name: Apis mellifera (Honeybee); NCBI TaxID: 7460;
ModificationList	monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-17 10:42:11	ID requested
1	2022-11-21 03:42:37	announced
⏵ 2	2023-11-14 08:16:31	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Apis mellifera

Formic acid

Varroa

parasite

Proteomics

Prof. Kevin Kavanagh
contact affiliation	Department of Biology, Maynooth University, Maynooth, Co. Kildare, Ireland.
contact email	kevin.kavanagh@mu.ie
lab head
Rachel Ward
contact affiliation	Maynooth Univeristy
contact email	rachel.ward.2015@mumail.ie
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026058
     1. Label: PRIDE project
     2. Name: Exposure of Apis mellifera to anti-Varroa destructor formic acid treatment induces significant proteomic alterations.