PXD026048

PXD026048 is an original dataset announced via ProteomeXchange.

Title	IP-MALDI-MS assay LOD for VP40 by targeting two peptide variants
Description	We developed a quantitative assay for carefully selected VP40 peptide variants. We selected two peptides, LGPGIPDHPLR from EBOV VP40 and LRPILLPGK from BDBV VP40 to generate pan-peptide variants antibodies. Potential structural difference in virion particles affect the efficiency of VP40 liberation during sample inactivation, and the sequence variations also affect the affinity of peptide against the pan-variants antibody. Therefore, we quantified the mass contents of VP40 in EBOV and SUDV VLPs and used the VLPs from each species to build the species-specific standard curves. This improves the accuracy of VP40 quantitation. The feasibility of using miniature mass spectrometer to collect the tandem MS spectra for both peptides was validated.
HostingRepository	PRIDE
AnnounceDate	2021-11-02
AnnouncementXML	Submission_2021-11-02_07:01:40.511.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qingbo Shu
SpeciesList	scientific name: Ebolavirus; NCBI TaxID: 186536;
ModificationList	No PTMs are included in the dataset
Instrument	autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-17 03:22:29	ID requested
⏵ 1	2021-11-02 07:01:40	announced

Dataset with its publication pending

submitter keyword: VP40, immunoprecipitation

Tony Hu
contact affiliation	Center for Cellular and Molecular Diagnostics, Department of Biochemistry and Molecular Biology, School of Medicine, Tulane University
contact email	tonyhu@tulane.edu
lab head
Qingbo Shu
contact affiliation	Tulane University
contact email	qshu@tulane.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026048
     1. Label: PRIDE project
     2. Name: IP-MALDI-MS assay LOD for VP40 by targeting two peptide variants