PXD026015

PXD026015 is an original dataset announced via ProteomeXchange.

Title	TMT-Based Quantitative Proteomic Analysis Reveals the Crucial Biological Pathways Involved in Brain Injury Induced by Chest Exposure to Shock Waves
Description	Long term chest blast exposure can lead to mental disorders, brain inflammation and oxidative stress in soldiers. However, the underlying mechanism of brain injury caused indirectly by chest blast remains unclear. It is urgent to find additional reliable biomarkers to reveal the intimate details of the pathogenesis of this phenomenon. We used iTRAQ labeling combined with LC-MS/MS to screen potential differentially expressed proteins in rat brain after chest blast at different time points. Meanwhile, we also used GO, KEGG, David and Cytoscape to analyze the proteomic profile and explore its pathogenesis. Moreover, Western blotting was used to verify the target proteins. Our data showed that, a total of 6,931 proteins were identified. A total of 255 differentially expressed proteins were identified, of which 43, 84, 52, 97 and 49 proteins were identified from brain tissues at 12h, 24h, 48h, 72h, and 1w after chest blast exposure, respectively. Bioinformatics analysis, including GO, COG, KEGG and STRING, further proved that brain damage caused by chest blast exposure affects many important biological processes and signal pathways, such as inflammation, cell adhesion, phagocytosis, neuronal and synaptic damage, oxidative stress and apoptosis. Moreover, Western blotting further confirmed that these differentially expressed proteins and signaling pathways were associated with brain damage caused by chest blast exposure. For the first time, we screened and verified the potential protein biomarkers of brain damage caused indirectly by chest blast, and provided a new target for the treatment of this disease.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_03:35:42.555.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Changci Tong
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-14 02:44:41	ID requested
⏵ 1	2022-02-17 03:35:43	announced

Tong C, Cong P, Liu Y, Shi X, Shi L, Mao S, Zhao Y, Hou M, Liu Y, Tandem Mass Tag-Based Quantitative Proteomic Analysis Reveals Pathways Involved in Brain Injury Induced by Chest Exposure to Shock Waves. Front Mol Neurosci, 14():688050(2021) [pubmed]

submitter keyword: Chest blast

brain injury

tandem mass spectrometry quantitative proteomics

inflammation

signaling pathways

Yunen Liu
contact affiliation	The Second Affiliated Hospital of Shenyang Medical College. The Veterans General Hospital of Liaoning Province
contact email	lye9901@163.com
lab head
Changci Tong
contact affiliation	Shenyang Medical College
contact email	cszx_tcc@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026015
     1. Label: PRIDE project
     2. Name: TMT-Based Quantitative Proteomic Analysis Reveals the Crucial Biological Pathways Involved in Brain Injury Induced by Chest Exposure to Shock Waves