PXD025995

PXD025995 is an original dataset announced via ProteomeXchange.

Title	Digestion of TSN2 and TSN89 synthetic peptides by proteasomes
Description	Proteasomes are large multi-subunit enzymes that act as the main producers of antigenic peptides presented at the cell surface to CD8+ T cells. They can simply cut proteins or ligate non-contiguous peptide fragments thereby generating novel sequences, i.e. spliced peptides via a process called proteasome-catalysed peptide splicing (PCPS). In order to benchmark methods for spliced peptide identification, we here perform in vitro digestions of TSN2 and TSN89 synthetic polypeptide substrates by K562-derived 20S proteasome followed by LC-MS/MS measurements using Orbitrap Fusion Lumos mass spectrometer. Michele Mishto, Head of the research group Molecular Immunology at King’s College London and the Francis Crick Institute, London (UK). Email: michele.mishto@kcl.ac.uk
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:55:53.214.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michele Mishto
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-13 03:30:11	ID requested
1	2022-12-19 01:30:10	announced
⏵ 2	2023-11-14 08:55:53	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Synthetic peptides, proteasome, digestion, LC-MS/MS

Juliane Liepe
contact affiliation	Head of research group Quantitative and Systems Biology; Max Planck Institute for Multidisciplinary Sciences; Goettingen; Germany
contact email	jliepe@mpinat.mpg.de
lab head
Michele Mishto
contact affiliation	King's College London
contact email	michele.mishto@kcl.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025995
     1. Label: PRIDE project
     2. Name: Digestion of TSN2 and TSN89 synthetic peptides by proteasomes