PXD025993

PXD025993 is an original dataset announced via ProteomeXchange.

Title	Proteomics of rat neonatal cardiac fibroblasts
Description	Gβγ subunits are involved in many different signalling processes in various compartments of the cell, including the nucleus. To gain insight into the functions of nuclear Gβγ, we investigated the functional role of Gβγ signalling in regulation of GPCR-mediated gene expression in primary rat neonatal cardiac fibroblasts. Following activation of the angiotensin II type I receptor in these cells, Gβγ dimers interact with RNA polymerase II (RNAPII). Our findings suggest that Gβ1γ recruitment to RNAPII negatively regulates the fibrotic transcriptional response, which can be overcome by strong fibrotic stimuli. In these specific proteomics experiments, we compared the differential protein abundance in Gβ1 knockdown and WT cardiac fibroblasts following angiotensin II treatment using a bottom-up data-dependent approach.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:46:31.937.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrew Bayne
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	impact II

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-13 03:29:56	ID requested
1	2023-05-10 13:19:19	announced
⏵ 2	2023-11-14 08:46:40	announced	2023-11-14: Updated project metadata.

Khan SM, Martin RD, Bayne A, P, é, trin D, Bourque K, Jones-Tabah J, Bouazza C, Blaney J, Lau J, Martins-Cannavino K, Gora S, Zhang A, MacKinnon S, Trieu P, Clarke PBS, Trempe JF, Tanny JC, H, é, bert TE, subunits colocalize with RNA polymerase II and regulate transcription in cardiac fibroblasts. J Biol Chem, 299(4):103064(2023) [pubmed]

submitter keyword: GNB1,rat cardiac fibroblasts, angiotensin II, bottom-up proteomics

Jean-Francois Trempe
contact affiliation	McGill University Department of Pharmacology and Therapeutics Montreal, QC
contact email	jeanfrancois.trempe@mcgill.ca
lab head
Andrew Bayne
contact affiliation	McGill University
contact email	andrew.bayne@mail.mcgill.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD025993

PRIDE project URI

• PRIDE
  1. PXD025993
     1. Label: PRIDE project
     2. Name: Proteomics of rat neonatal cardiac fibroblasts