PXD025989

PXD025989 is an original dataset announced via ProteomeXchange.

Title	Metabolic characterization of hepatic disease patients with hepatitis B virus DNA replication by untargeted metabolomics
Description	Purpose: Worldwide, over 350 million people are chronically infected with the hepatitis B virus (HBV) and are at increased risk of developing progressive hepatic diseases (hepatitis, cirrhosis or liver cancer). Still, the metabolic processes operational during the distinct clinical phases of a chronic HBV infection to liver disease remains unexplored. Thus, the aim of our study was to determine the serum metabolic characteristics of hepatic disease patients with chronic hepatitis B in active and inactive stage and study the influence of active and inactive stage of the chronic hepatitis B virus on the development of liver cancer by untargeted metabolomics. Method: To investigate this, we conducted an untargeted metabolomics approach to determine the metabolic characteristics of 199 human serum samples from hepatic disease patients in active and inactive chronic HBV via UHPLC-Q-TOF/MS, and patients with HBV in active or inactive stage were grouped based on their serum levels of HBV DNA and HBV envelope antigen (HBeAg). Principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and volcano plot were used for multivariate analysis. Pathway analysis were conducted according to p-values from pathway enrichment analysis and pathway impact values from pathway topology analysis. In addition, commercial databases were used to identify metabolites from significantly altered metabolic pathways. All samples were analyzed for liver function tests indicated the levels of certain enzymes. Results: Our data provide insight into the metabolic dysregulation experienced during active or inactive stage of the HBV on the development of liver disease, and analysis of serum metabolomic profiles indicated that most of the altered metabolic pathways for chronic hepatitis and cirrhosis patients with active or inactive stage of the HBV were associated with D-Glutamine and D-glutamate metabolism, indicating a strong link to HBV replication (p < 0.05). In addition, liver function analysis showed the enzymes gamma-glutamyl transferase (GGT) were increased concomitant to the progression of hepatitis to cirrhosis induced by chronic HBV (p < 0.05).
HostingRepository	iProX
AnnounceDate	2021-05-13
AnnouncementXML	Submission_2022-05-31_20:43:41.495.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lin Yu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-12 20:22:45	ID requested
1	2021-05-12 20:23:12	announced
⏵ 2	2022-05-31 20:43:42	announced	2022-06-01: Upload publication information.

Yu L, Zeng Z, Tan H, Feng Q, Zhou Q, Hu J, Li Y, Wang J, Yang W, Feng J, Xu B, Significant metabolic alterations in patients with hepatitis B virus replication observed via serum untargeted metabolomics shed new light on hepatitis B virus infection. J Drug Target, 30(4):442-449(2022) [pubmed]

submitter keyword: chronic hepatitis, cirrhosis, liver cancer, HBV infection, HBV DNA replication, untargeted metabolomics.

Jiafu Feng
contact affiliation	Departmant of Clinical Laboratory, Mianyang Central Hospital, School of Medicine, University of Electronic Science and Technology of China
contact email	jiafufeng@foxmail.com
lab head
Lin Yu
contact affiliation	Mianyang Central Hospital, School of Medicine, University of Electronic Science and Technology of China
contact email	xiaoyulin918@163.com
dataset submitter

iProX dataset URI