PXD025986

PXD025986 is an original dataset announced via ProteomeXchange.

Title	Human PDR Vitreous Variability Study, TMT-MS
Description	Control (n=27) and proliferative diabetic retinopathy (n=23) vitreous samples were treated as biologically distinct individuals or pooled together and aliquoted into technical replicates. Quantitative mass spectrometry with tandem mass tag labeling was used to identify proteins in individual or pooled control samples to determine technical and biological variability. To determine effect size and perform power analysis, control and proliferative diabetic retinopathy samples were analyzed across four 10plexes. Pooled samples were used to normalize the data across plexes and generate a single data matrix for downstream analysis.
HostingRepository	PRIDE
AnnounceDate	2022-02-14
AnnouncementXML	Submission_2022-02-14_02:29:05.380.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sarah Weber
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-12 08:56:01	ID requested
⏵ 1	2022-02-14 02:29:06	announced

Weber SR, Zhao Y, Ma J, Gates C, da Veiga Leprevost F, Basrur V, Nesvizhskii AI, Gardner TW, Sundstrom JM, A validated analysis pipeline for mass spectrometry-based vitreous proteomics: new insights into proliferative diabetic retinopathy. Clin Proteomics, 18(1):28(2021) [pubmed]

submitter keyword: human, vitreous, proliferative diabetic retinopathy, TMT-MS

Jeffrey M. Sundstrom
contact affiliation	Department of Ophthalmology, Penn State College of Medicine
contact email	jsundstrom@pennstatehealth.psu.edu
lab head
Sarah Weber
contact affiliation	Penn State College of Medicine
contact email	srw5267@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD025986

PRIDE project URI

• PRIDE
  1. PXD025986
     1. Label: PRIDE project
     2. Name: Human PDR Vitreous Variability Study, TMT-MS