PXD025967

PXD025967 is an original dataset announced via ProteomeXchange.

Title	Deciphering the O-glycosylation of HKU1 Spike Protein with the Dual-Functional Hydrophilic Interaction Chromatography Materials
Description	HKU1 is a human betacoronavirus and infects host cells via highly glycosylated spike protein (S). At present, N-glycosylation of HKU1 S has been reported, however, little is known about its O-glycosylation, which hinders an in-depth understanding of its biological functions. Herein, a comprehensive study of O-glycosylation of HKU1 S was carried out based on dual-functional histidine-bonded silica materials (HBS). The enrichment method for O-glycopeptides with HBS was developed and validated using standard proteins. The application of the developed method to HKU1 S1 subunit resulted in 61 novel O-glycosylation sites, among which 56% were predicted to be exposed on protein outer surface. Moreover, the O-linked glycans and their abundance on each HKU1 S1 site were also analyzed. The obtained O-glycosylation dataset would provide valuable insights for the understanding of the structure of HKU1 S.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_03:17:39.354.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD025967
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dongmei Fu
SpeciesList	scientific name: Human coronavirus HKU1 (isolate N1); NCBI TaxID: 443239;
ModificationList	complex glycosylation
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-12 01:45:48	ID requested
⏵ 1	2022-02-17 03:17:40	announced

Cui Y, Dong X, Zhang X, Chen C, Fu D, Li X, Liang X, Deciphering the O-Glycosylation of HKU1 Spike Protein With the Dual-Functional Hydrophilic Interaction Chromatography Materials. Front Chem, 9():707235(2021) [pubmed]

submitter keyword: HKU1, spike glycoprotein, enrichment, O-glycosylation sites, O-glycosylation abundance

Dongmei Fu
contact affiliation	School of Bioengineering, Dalian Polytechnic University
contact email	dongmeifu@163.com
lab head
Dongmei Fu
contact affiliation	School of Bioengineering, Dalian Polytechnic University,
contact email	dongmeifu@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD025967

PRIDE project URI

• PRIDE
  1. PXD025967
     1. Label: PRIDE project
     2. Name: Deciphering the O-glycosylation of HKU1 Spike Protein with the Dual-Functional Hydrophilic Interaction Chromatography Materials