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PXD025965

PXD025965 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAmaranthus hypochondriacus seeds as a rich source of cysteine rich bioactive peptides
DescriptionAmaranthus hypochondriacus is a highly nutritious alternative grain native to Central and South America but consumed worldwide. Increased interest in A. hypochondriacus has driven characterization of bioactive secondary metabolites and their impact on the human body. A. hypochondriacus seeds are known to contain bioactive small molecules but there is a dearth of knowledge regarding endogenous bioactive peptides, especially cysteine-rich peptides (CRPs) which may be resistant to human digestion. Here, 89 CRPs were predicted in silico from A. hypochondriacus, 13 of which were detected in a seed extract via bottom-up proteomics, providing direct evidence for the translation of snakins, hevein-like peptides, defensins, lipid transfer proteins, and α-hairpinins. Mature forms of four novel and two known CRPs were molecularly characterized via top-down mass spectrometry. Four peptides demonstrated resistance to in vitro gastrointestinal digestion, suggesting that A. hypochondriacus CRPs may exhibit bioactivity after consumption and should be prioritized for further characterization.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_09:02:40.671.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLeslie Hicks
SpeciesList scientific name: Amaranthus hypochondriacus; NCBI TaxID: 28502;
ModificationListacetylated residue; iodoacetamide derivatized residue
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-05-12 01:45:42ID requested
12023-07-20 10:51:42announced
22023-11-14 09:02:41announced2023-11-14: Updated project metadata.
Publication List
Moyer TB, Schug WJ, Hicks LM, Amaranthus hypochondriacus seeds as a rich source of cysteine rich bioactive peptides. Food Chem, 377():131959(2022) [pubmed]
Keyword List
submitter keyword: peptidomics, proteomics, alternative grain,Amaranthus hypochondriacus, mass spectrometry, cysteine rich peptides
Contact List
Leslie M. Hicks
contact affiliationUniversity of North Carolina at Chapel Hill, Department of Chemistry
contact emaillmhicks@unc.edu
lab head
Leslie Hicks
contact affiliationUniversity of North Carolina at Chapel Hill
contact emaillmhicks@unc.edu
dataset submitter
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Dataset FTP location
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