PXD025965 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Amaranthus hypochondriacus seeds as a rich source of cysteine rich bioactive peptides |
Description | Amaranthus hypochondriacus is a highly nutritious alternative grain native to Central and South America but consumed worldwide. Increased interest in A. hypochondriacus has driven characterization of bioactive secondary metabolites and their impact on the human body. A. hypochondriacus seeds are known to contain bioactive small molecules but there is a dearth of knowledge regarding endogenous bioactive peptides, especially cysteine-rich peptides (CRPs) which may be resistant to human digestion. Here, 89 CRPs were predicted in silico from A. hypochondriacus, 13 of which were detected in a seed extract via bottom-up proteomics, providing direct evidence for the translation of snakins, hevein-like peptides, defensins, lipid transfer proteins, and α-hairpinins. Mature forms of four novel and two known CRPs were molecularly characterized via top-down mass spectrometry. Four peptides demonstrated resistance to in vitro gastrointestinal digestion, suggesting that A. hypochondriacus CRPs may exhibit bioactivity after consumption and should be prioritized for further characterization. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_09:02:40.671.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Leslie Hicks |
SpeciesList | scientific name: Amaranthus hypochondriacus; NCBI TaxID: 28502; |
ModificationList | acetylated residue; iodoacetamide derivatized residue |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-05-12 01:45:42 | ID requested | |
1 | 2023-07-20 10:51:42 | announced | |
⏵ 2 | 2023-11-14 09:02:41 | announced | 2023-11-14: Updated project metadata. |
Publication List
Moyer TB, Schug WJ, Hicks LM, Amaranthus hypochondriacus seeds as a rich source of cysteine rich bioactive peptides. Food Chem, 377():131959(2022) [pubmed] |
Keyword List
submitter keyword: peptidomics, proteomics, alternative grain,Amaranthus hypochondriacus, mass spectrometry, cysteine rich peptides |
Contact List
Leslie M. Hicks |
contact affiliation | University of North Carolina at Chapel Hill, Department of Chemistry |
contact email | lmhicks@unc.edu |
lab head | |
Leslie Hicks |
contact affiliation | University of North Carolina at Chapel Hill |
contact email | lmhicks@unc.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD025965
- Label: PRIDE project
- Name: Amaranthus hypochondriacus seeds as a rich source of cysteine rich bioactive peptides