PXD025965

PXD025965 is an original dataset announced via ProteomeXchange.

Title	Amaranthus hypochondriacus seeds as a rich source of cysteine rich bioactive peptides
Description	Amaranthus hypochondriacus is a highly nutritious alternative grain native to Central and South America but consumed worldwide. Increased interest in A. hypochondriacus has driven characterization of bioactive secondary metabolites and their impact on the human body. A. hypochondriacus seeds are known to contain bioactive small molecules but there is a dearth of knowledge regarding endogenous bioactive peptides, especially cysteine-rich peptides (CRPs) which may be resistant to human digestion. Here, 89 CRPs were predicted in silico from A. hypochondriacus, 13 of which were detected in a seed extract via bottom-up proteomics, providing direct evidence for the translation of snakins, hevein-like peptides, defensins, lipid transfer proteins, and α-hairpinins. Mature forms of four novel and two known CRPs were molecularly characterized via top-down mass spectrometry. Four peptides demonstrated resistance to in vitro gastrointestinal digestion, suggesting that A. hypochondriacus CRPs may exhibit bioactivity after consumption and should be prioritized for further characterization.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:02:40.671.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Leslie Hicks
SpeciesList	scientific name: Amaranthus hypochondriacus; NCBI TaxID: 28502;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-12 01:45:42	ID requested
1	2023-07-20 10:51:42	announced
⏵ 2	2023-11-14 09:02:41	announced	2023-11-14: Updated project metadata.

Moyer TB, Schug WJ, Hicks LM, Amaranthus hypochondriacus seeds as a rich source of cysteine rich bioactive peptides. Food Chem, 377():131959(2022) [pubmed]

submitter keyword: peptidomics, proteomics, alternative grain,Amaranthus hypochondriacus, mass spectrometry, cysteine rich peptides

Leslie M. Hicks
contact affiliation	University of North Carolina at Chapel Hill, Department of Chemistry
contact email	lmhicks@unc.edu
lab head
Leslie Hicks
contact affiliation	University of North Carolina at Chapel Hill
contact email	lmhicks@unc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD025965

PRIDE project URI

• PRIDE
  1. PXD025965
     1. Label: PRIDE project
     2. Name: Amaranthus hypochondriacus seeds as a rich source of cysteine rich bioactive peptides