PXD025961

PXD025961 is an original dataset announced via ProteomeXchange.

Title	A homeobox7 transcription factor from Mesembryanthemum crystallinum enhanced salt tolerance
Description	Mesembryanthemum crystallinum (common ice plant) is a facultative halophyte species, which has adapted to extreme conditions. In this study, we cloned a McHB7 transcription factor gene from the ice plant. The expression of McHB7 was significantly induced by 500 mM NaCl and it reached the peak under salt treatment for 7 days. The McHB7 protein was targeted to the nucleus. Overexpression of McHB7 gene in ice plant leaves by Agrobacterium-mediated transformation led to 25 times more McHB7 transcripts than the non-transformed control wild type (WT). After 500 mM NaCl treatment for 7 days, SOD, POD activities and water content of the transgenic plants were significantly higher than WT, while MDA content was decreased in the transgenic plants. Proteomics results showed that a total of 1082 and 1072 proteins were profiled under control and salt treatment, respectively. 22 (2%) and 11 (1%) proteins were uniquely identified under control and salt stress conditions, respectively. Among these 11 proteins, 7 were increased and 4 were decreased. Most identified proteins involved in the processes of regulation of biological, transporter and catalytic activity, biosynthesis of secondary metabolties and response to stimulus were significantly increased in the McHB7 overexpression ice plants under high salinity. All the results demonstrate that the McHB7 transcription factor plays a positive role in improving plant salt tolerance.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_03:21:20.612.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xuemei Zhang
SpeciesList	scientific name: Mesembryanthemum crystallinum; NCBI TaxID: 3544;
ModificationList	carbamoylated residue; monohydroxylated residue
Instrument	Q Exactive HF; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-11 23:48:36	ID requested
⏵ 1	2022-02-17 03:21:21	announced

Zhang X, Tan B, Zhu D, Dufresne D, Jiang T, Chen S, . Int J Mol Sci, 22(12):(2021) [pubmed]

submitter keyword: Mesembryanthemum crystallinum

McHB7

transcription factor

salt treatment

proteomics

Sixue Chen
contact affiliation	Department of Biology, Genetics Institute, University of Florida, Gainesville, FL 32610, USA
contact email	schen@ufl.edu
lab head
Xuemei Zhang
contact affiliation	University of Florida
contact email	zhangxuemei199111@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025961
     1. Label: PRIDE project
     2. Name: A homeobox7 transcription factor from Mesembryanthemum crystallinum enhanced salt tolerance