PXD025959

PXD025959 is an original dataset announced via ProteomeXchange.

Title	Coordinated control of the Aurora B abscission checkpoint by PKCε complex assembly, midbody recruitment and retention
Description	The N371 site on PKCe was mutated to TAG in an expression plasmid. This was co-transfected in a 1:1 ratio with a plasmid that encodes both an aaRS optimised to recognise AbK, and the tRNAPyl which recognises the UAG codon.
HostingRepository	PRIDE
AnnounceDate	2021-11-03
AnnouncementXML	Submission_2021-11-03_05:08:03.229.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD025959
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Steven Lynham
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-11 23:39:57	ID requested
⏵ 1	2021-11-03 05:08:04	announced

Watson L, Soliman TN, Davis K, Kelly J, Lockwood N, Yang X, Lynham S, Scott JD, Crossland V, McDonald NQ, Mann DJ, Armstrong A, Eggert U, Parker PJ, complex assembly, midbody recruitment and retention. Biochem J, 478(12):2247-2263(2021) [pubmed]

submitter keyword: 14-3-3 PKCe, phosphorylation, mass spectrometry

Prof. Peter Parker
contact affiliation	King’s College London and the Francis Crick Institute
contact email	peter.parker@kcl.ac.uk
lab head
Steven Lynham
contact affiliation	King's College London
contact email	steven.lynham@kcl.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD025959

PRIDE project URI

• PRIDE
  1. PXD025959
     1. Label: PRIDE project
     2. Name: Coordinated control of the Aurora B abscission checkpoint by PKCε complex assembly, midbody recruitment and retention