PXD025935

PXD025935 is an original dataset announced via ProteomeXchange.

Title	Palm oil-rich diet affects murine liver proteome and S-palmitoylome
Description	Palmitic acid (C16:0) is the most abundant saturated fatty acid in animals serving as a substrate in lipid synthesis and β-oxidation, and in the modification of proteins called palmitoylation. The influence of dietary palmitic acid on protein palmitoylation in the context of metabolic processes remains largely unknown. In this study we performed high-throughput proteomic analyses of the liver membrane fraction in the mouse to examine the influence of a palm oil-rich diet on the level and S-palmitoylation of proteins. For this purpose, mice were fed for 4 or 12 weeks a diet containing 19.1% of palm oil in addition to 4% soybean oil (45% kcal from fat) while 4% soybean oil (10% kcal from fat) was the only fat source in the control diet. Liver functioning and pro-inflammatory responses of the liver and peritoneal macrophages as well as the input of protein S-palmitoylation to these aspects were assessed in parallel. We found that the diet rich in palm oil induced transient accumulation of C16:0 and C18:1 fatty acids in murine liver leading to changes of the level and S-palmitoylation of numerous proteins involved in liver metabolism and selected innate immune responses. The relatively mild negative impact of such diet on liver functioning can be attributed to a lower bioavailability of palm oil-derived C16:0 vs. that of C18:1 and the efficiency of mechanisms preventing liver injury, including dynamic protein S-palmitoylation.
HostingRepository	PRIDE
AnnounceDate	2021-12-14
AnnouncementXML	Submission_2021-12-21_12:45:49.852.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD025935
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Agata Malinowska
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	methylthiolated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-11 03:35:08	ID requested
1	2021-12-14 02:26:38	announced
⏵ 2	2021-12-21 12:45:50	announced	2021-12-21: Updated publication reference for PubMed record(s): 34884899.

Ziemli, ń, ska E, Soboci, ń, ska J, Ś, wi, ą, tkowska A, Hromada-Judycka A, Traczyk G, Malinowska A, Ś, widerska B, Mietelska-Porowska A, Ciesielska A, Kwiatkowska K, -Palmitoylome. Int J Mol Sci, 22(23):(2021) [pubmed]

submitter keyword: S-palmitoylation, high-fat diet, palm oil, neutrophil degranulation, calfacilitin/TLCD1, mass spectrometry

Michal Dadlez
contact affiliation	Mass Spectrometry Laboratory, Institute of Biochemistry and Biophysics, Polish Academy of Sciences
contact email	michald@ibb.waw.pl
lab head
Agata Malinowska
contact affiliation	IBB PAS
contact email	esme@ibb.waw.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/12/PXD025935

PRIDE project URI

• PRIDE
  1. PXD025935
     1. Label: PRIDE project
     2. Name: Palm oil-rich diet affects murine liver proteome and S-palmitoylome