PXD025931

PXD025931 is an original dataset announced via ProteomeXchange.

Title	The protein interaction landscape of breast cancer
Description	Cancers have been associated with a diverse array of genomic alterations. To understand such alterations in breast invasive carcinoma at the level of cellular mechanisms, we have applied affinity-purification mass spectrometry to delineate comprehensive biophysical interaction networks for 40 frequently altered breast cancer proteins across three human breast cell lines, providing a resource of context-specific and shared protein-protein interaction networks. These networks interconnect and enrich for common and rare cancer mutations, and are substantially rewired by mutations. Our analysis identifies PIK3CA-interacting proteins which repress AKT signaling, and UBE2N emerges as a BRCA1 interactor predictive of clinical response to PARP inhibition. We also show that Spinophilin interacts with and dephosphorylates BRCA1 to promote DNA double-strand break repair. Thus, cancer protein interaction landscapes provide a framework for recognizing oncogenic drivers and drug vulnerabilities.
HostingRepository	PRIDE
AnnounceDate	2021-11-02
AnnouncementXML	Submission_2021-11-02_04:59:56.979.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Minkyu Kim
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitination signature dipeptidyl lysine; phosphorylated residue
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-11 02:39:05	ID requested
⏵ 1	2021-11-02 04:59:57	announced

Kim M, Park J, Bouhaddou M, Kim K, Rojc A, Modak M, Soucheray M, McGregor MJ, O'Leary P, Wolf D, Stevenson E, Foo TK, Mitchell D, Herrington KA, Mu, ñ, oz DP, Tutuncuoglu B, Chen KH, Zheng F, Kreisberg JF, Diolaiti ME, Gordan JD, Copp, é JP, Swaney DL, Xia B, van 't Veer L, Ashworth A, Ideker T, Krogan NJ, A protein interaction landscape of breast cancer. Science, 374(6563):eabf3066(2021) [pubmed]

submitter keyword: human, breast cancer, ub, ph

Nevan Krogan
contact affiliation	Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA
contact email	minkyu.kim@ucsf.edu
lab head
Minkyu Kim
contact affiliation	University of California San Francisco
contact email	minkyu.kim@ucsf.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD025931

PRIDE project URI

• PRIDE
  1. PXD025931
     1. Label: PRIDE project
     2. Name: The protein interaction landscape of breast cancer