PXD025929

PXD025929 is an original dataset announced via ProteomeXchange.

Title	A regulatory network comprising let-7 miRNA and SMUG1 is associated with good prognosis in breast cancer survival
Description	Cytosine deamination to uracil in DNA leads to mutations if the DNA lesion is not corrected. Base excision repair (BER) initiated by single-strand selective uracil-DNA glycosylase 1 (SMUG1) primarily recognizes uracil and oxidized pyrimidines, and processes them to restore the correct bases. SMUG1 status has been associated with cancer risk and therapeutic response as high levels of SMUG1 were observed in breast carcinomas and other cancer types. The role of SMUG1 in breast cancer is not completely understood. Here, we define a bad prognosis signature for SMUG1 interactors in different cancers by mapping out a SMUG1 interaction network. Survival analyses of patient outcomes show that high expression of genes in the bad prognosis network correlates with lower survival probability in breast cancer. Interestingly, bioinformatics analyses suggested let-7b-5p microRNA as one of the upstream regulators of SMUG1 interactors. Expression of SMUG1 and let-7b-5p are negatively correlated in breast cancer and we observed an inhibitory auto-regulatory loop between SMUG1 and let-7b-5p in MCF-7 cells. Hence, SMUG1 is a key player connecting RNA and DNA metabolism with regulation of gene expression.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:23:31.848.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bernd Thiede
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-11 02:38:52	ID requested
1	2023-03-09 04:22:46	announced
⏵ 2	2023-11-14 08:23:32	announced	2023-11-14: Updated project metadata.

Lirussi L, Ayyildiz D, Liu Y, Montaldo NP, Carracedo S, Aure MR, Jobert L, Tekpli X, Touma J, Sauer T, Dalla E, Kristensen VN, Geisler J, Piazza S, Tell G, Nilsen H, A regulatory network comprising let-7 miRNA and SMUG1 is associated with good prognosis in ER+ breast tumours. Nucleic Acids Res, 50(18):10449-10468(2022) [pubmed]

submitter keyword: SMUG1, breast cancer, DNA repair, RNA and ribonucleoprotein biogenesis, hsa-let-7b-5p, modified bases

Bernd Thiede
contact affiliation	University of Oslo
contact email	bernd.thiede@ibv.uio.no
lab head
Bernd Thiede
contact affiliation	University of Oslo
contact email	bernd.thiede@ibv.uio.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD025929

PRIDE project URI

• PRIDE
  1. PXD025929
     1. Label: PRIDE project
     2. Name: A regulatory network comprising let-7 miRNA and SMUG1 is associated with good prognosis in breast cancer survival