PXD025926

PXD025926 is an original dataset announced via ProteomeXchange.

Title	Non-Refoldability is Pervasive Across the E. coli Proteome
Description	Decades of protein folding research have focused on a small, privileged subset of proteins that can reversibly refold upon denaturation. However, these proteins are not necessarily representative of the complexity of natural proteomes, which consist of many proteins with more sophisticated architectures. Here, we introduce an experimental approach to probe the refolding of whole proteomes. To accomplish this, we first unfold and refold E. coli lysates, and then interrogate the resulting protein structures usin a permissive enzyme that preferentially cleaves at more flexible regions. Using mass spectrometry, we analyze the digestion patterns to globally assess structural differences between native and “refolded” proteins. These studies reveal that many soluble proteins are incapable of navigating back to their native structures upon chemical denaturation, highlighting the role of exogenous factors and processes such as molecular chaperones or co-translational folding for efficient protein folding.
HostingRepository	PRIDE
AnnounceDate	2021-07-12
AnnouncementXML	Submission_2021-07-11_21:47:23.759.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephen Fried
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-10 23:22:22	ID requested
⏵ 1	2021-07-11 21:47:24	announced

Dataset with its publication pending

submitter keyword: Protein Refolding, Proteomics

Stephen Fried
contact affiliation	Johns Hopkins University, Chemistry Department, Fried Lab, USA
contact email	sdfried@jhu.edu
lab head
Stephen Fried
contact affiliation	Johns Hopkins University
contact email	sdfried@jhu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025926
     1. Label: PRIDE project
     2. Name: Non-Refoldability is Pervasive Across the E. coli Proteome