PXD025924

PXD025924 is an original dataset announced via ProteomeXchange.

Title	Campylobacter gene regulation for persistance and survival
Description	The pathogenic bacterium Campylobacter jejuni is the leading cause of bacterial foodborne gastroenteritis worldwide yet it does not grow in the aerobic environment. The paralogues RrpA and RrpB which are members of MarR family of DNA binding proteins have been shown to be important for the survival of C. jejuni under aerobic and redox stress. We report that RrpA is a positive regulator of mdaB, encoding a flavin-dependent quinone reductase. MdaB confers protection to the cell from redox stress mediated by structurally diverse quinones. RrpB negatively regulates the expression of nfrA (Cj1555c), a flavin reductase. NfrA reduces riboflavin at a much higher rate than flavin mononucleotide (FMN),suggesting exogenous free flavins are the natural substrate. Enzymatic activity of MdaB and NfrA towards their substrates revealed both reductases preferred NADPH as an electron donor. DNA-binding and post translational modification analyses showed that the mechanism of RrpA and RrpB DNA binding is likely a cysteine-based redox switch. Complete genome sequences analysis indicated that MdaB is predominant in Campylobacter spp. and the related Helicobacter spp., whilst NfrA is more often found in C. jejuni strains. Quinones and flavins are antimicrobial redox cycling agents secreted by a wide range of cell-types that can form damaging superoxide by one-electron reactions. We propose that MdaB and NfrA production allows a two-electron reduction mechanism to the less toxic quinol forms. These enzymes thus aid the survival and persistence of C. jejuni in the face of toxic compounds from competing microbes.
HostingRepository	PRIDE
AnnounceDate	2021-11-02
AnnouncementXML	Submission_2021-11-02_04:04:23.306.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD025924
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Steven Lynham
SpeciesList	scientific name: Campylobacter jejuni subsp. jejuni 81-176-UMCW9; NCBI TaxID: 1437113;
ModificationList	L-cysteine sulfinic acid; L-cysteic acid (L-cysteine sulfonic acid)
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-10 23:16:26	ID requested
⏵ 1	2021-11-02 04:04:24	announced

Nasher F, Taylor AJ, Elmi A, Lehri B, Ijaz UZ, Baker D, Goram R, Lynham S, Singh D, Stabler R, Kelly DJ, Gundogdu O, Wren BW, MdaB and NfrA, Two Novel Reductases Important in the Survival and Persistence of the Major Enteropathogen Campylobacter jejuni. J Bacteriol, 204(1):e0042121(2022) [pubmed]

submitter keyword: RrpA, RrpB, Redox cycling

Dr Fauzy Nasher
contact affiliation	Department of Infection Biology, Faculty of Infectious and Tropical Diseases, London School of Hygeine & Tropical Medicine
contact email	Fauzy.Nasher1@lshtm.ac.uk
lab head
Steven Lynham
contact affiliation	King's College London
contact email	steven.lynham@kcl.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025924
     1. Label: PRIDE project
     2. Name: Campylobacter gene regulation for persistance and survival