PXD025921

PXD025921 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteomic analysis of the survival effect of TPO receptor agonist Romiplostim treatment in mice exposed to a lethal dose of radiation
Description	Background: The thrombopoietin receptor agonist romiplostim (RP) is a therapeutic agent for immune thrombocytopenia that can achieve complete survival in mice exposed to a lethal dose of ionizing radiation. The estimated mechanism of the radio-protective/mitigative effects of RP has been proposed; however, the detailed mechanism of action remains unclear. The present study aimed to elucidate the mechanism of the radio-protective/mitigative effects of RP, the fluctuation of protein in the blood was analyzed by proteomics. Methods: Peripheral blood was harvested from the fundus of mice treated with non-total body irradiation (TBI) and 7 Gy TBI with or without the administration of RP on days 10 and 18 after TBI. Serum prepared from each treated mouse was analyzed using a Liquid Chromatograph-tandem Mass Spectrometer. Results: Eleven proteins were identified by proteomics methods from 269 analyzed proteins detected in mice exposed to a lethal dose of TBI. Many proteins were found to be involved in cell death and liver damage. Conclusions: It was suggested that the effect of RP in reducing radiation damage may be largely related to the suppression of cell death and liver damage.
HostingRepository	jPOST
AnnounceDate	2021-05-12
AnnouncementXML	Submission_2022-09-18_02:43:41.255.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yota Tatara
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine; N6-acetyl-L-lysine; alpha-amino acetylated residue; 2-pyrrolidone-5-carboxylic acid (Gln); 2-pyrrolidone-5-carboxylic acid (Glu); L-methionine sulfoxide; phosphorylated L-histidine; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine
Instrument	TripleTOF 6600

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-10 22:45:05	ID requested
1	2021-05-12 03:14:54	announced
⏵ 2	2022-09-18 02:43:42	announced	2022-09-18: Updated FTP location.

Publication List

Nishida T, Yamaguchi M, Tatara Y, Kashiwakura I, Proteomic changes by radio-mitigative thrombopoietin receptor agonist romiplostim in the blood of mice exposed to lethal total-body irradiation. Int J Radiat Biol, 96(9):1125-1134(2020) [pubmed]

Nishida T, Yamaguchi M, Tatara Y, Kashiwakura I, Proteomic changes by radio-mitigative thrombopoietin receptor agonist romiplostim in the blood of mice exposed to lethal total-body irradiation. Int J Radiat Biol, 96(9):1125-1134(2020) [pubmed]

Keyword List

submitter keyword: mouse, serum

submitter keyword: mouse, serum

Contact List

Ikuo Kashiwakura
lab head
Yota Tatara
contact affiliation	Hirosaki University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001161/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001161/