PXD025919
PXD025919 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | proteome analyses reveal protein metabolism in Lactobacillus helveticus |
| Description | Lactobacillus helveticus is a homofermentative lactic acid bacterium. It is widely used in the fabrication of Swiss cheese and other dairy products. The aim of this study was to elucidate the mechanism by which L. helveticus utilizes protein. L. helveticus CICC22171 were cultured in two different media with various nitrogen sources. The control contained 20 basic amino acids while the experimental medium contained casein. De novo transcriptome and iTRAQ proteome analyses were applied to determine how L. helveticus utilizes protein. The casein underwent extracellular hydrolysis via ATP-binding cassette (ABC) transporter upregulation and Mn2+-associated cell envelope proteinase (CEP) downregulation. Sigma factors and EF-Tu were upregulated and Mg2+ was reduced in bacteria to accommodate DNA transcription and protein translation in preparation for proteolysis. Hydrolase activity was upregulated to digest intracellular polypeptides and control endopeptidase genes. In these bacteria, casein utilization affected glycolysis, trehalose phosphotransferase system (PTS), and key factors associated with aerobic respiration and reduced glucose consumption. |
| HostingRepository | iProX |
| AnnounceDate | 2021-05-11 |
| AnnouncementXML | Submission_2021-08-03_01:36:29.722.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Mengfan Xu |
| SpeciesList | scientific name: uncultured Lactobacillus sp.; NCBI TaxID: 153152; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2021-05-10 20:58:10 | ID requested | |
| 1 | 2021-05-31 23:31:10 | announced | |
| ⏵ 2 | 2021-08-03 01:36:30 | announced | 2021-08-03: Update publication information. |
Publication List
| Xu M, Hu S, Wang Y, Wang T, Dziugan P, Zhang B, Zhao H, CICC22171. Front Microbiol, 12():635685(2021) [pubmed] |
Keyword List
| submitter keyword: proteome, Lactobacillus helveticus, protein metabolism |
Contact List
| Hongfei Zhao | |
|---|---|
| contact affiliation | Beijing forestry university |
| contact email | zhaohf518@163.com |
| lab head | |
| Mengfan Xu | |
| contact affiliation | Beijing forestry university |
| contact email | xumengfan1234@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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