PXD025915

PXD025915 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of bile acids modulation on rat colonic expression of water, electrolyte and drug transporters
Description	Bile acid diarrhoea is a chronic condition with increased delivery of bile acids to the colon causing diarrhoea. In this study we used a rodent model of bile acid malabsorption to investigate the general impact on increased levels of bile acids on mRNA- and protein-expression in colon epithelial cells. Twelve male Wistar Munich rats were housed in metabolic cages and fed control or bile acid-mixed (1% w/w) diets for ten days. After euthanasia, colonic epithelial cells were isolated using Ca2+ chelation and processed for mass spectrometry-based proteomics to quantify changes in protein expressions.
HostingRepository	PRIDE
AnnounceDate	2021-11-03
AnnouncementXML	Submission_2021-11-03_05:54:19.276.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qi Wu
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-10 05:58:27	ID requested
⏵ 1	2021-11-03 05:54:19	announced

Yde J, Wu Q, Borg JF, Fenton RA, Moeller HB, A systems-level analysis of bile acids effects on rat colon epithelial cells. Am J Physiol Gastrointest Liver Physiol, 322(1):G34-G48(2022) [pubmed]

submitter keyword: proteomics, bile acid, rat, colon, transporter

Hanne B. Møller
contact affiliation	Department of Biomedicine, Aarhus University
contact email	hbmo@biomed.au.dk
lab head
Qi Wu
contact affiliation	Department of Biomedicine, Aarhus University
contact email	qi.wu@biomed.au.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD025915

PRIDE project URI

• PRIDE
  1. PXD025915
     1. Label: PRIDE project
     2. Name: Proteomics analysis of bile acids modulation on rat colonic expression of water, electrolyte and drug transporters